Solution structure and interaction of the carboxyl-terminal domain of the Escherichia coli RNA polymerase α subunit as studied by NMR NMRによる大腸菌RNAポリメラーゼαサブユニットC末端ドメインの構造と相互作用の研究
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著者
書誌事項
- タイトル
-
Solution structure and interaction of the carboxyl-terminal domain of the Escherichia coli RNA polymerase α subunit as studied by NMR
- タイトル別名
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NMRによる大腸菌RNAポリメラーゼαサブユニットC末端ドメインの構造と相互作用の研究
- 著者名
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田, 栄浩
- 著者別名
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デン, ヨンホー
- 学位授与大学
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大阪大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第5437号
- 学位授与年月日
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1996-03-25
注記・抄録
博士論文
目次
- Contents / (0004.jp2)
- General Introduction / p1 (0007.jp2)
- (a)Aim and Summary of This Thesis / p2 (0008.jp2)
- (b)Carboxyl-terminal Domain of Escherichia coli RNA Polymerase αSubunit (αCTD) / p2 (0008.jp2)
- (c)Protein Structure Determination with Multi-dimensional NMR Spectroscopy / p5 (0011.jp2)
- References / p9 (0015.jp2)
- Chapter 1.Expression and Purification of the COOH-terminal Domain of RNA Polymerase α Subunit of E.coli(αCTD) / p10 (0018.jp2)
- (a)Abstract / p11 (0019.jp2)
- (b)Introduction / p11 (0019.jp2)
- (c)Experimental Procedure / p11 (0019.jp2)
- (d)Results and Discussion / p12 (0020.jp2)
- References / p14 (0022.jp2)
- Chapter 2.Assignment of Nuclear Magnetic Resonances of αCTD / p16 (0030.jp2)
- (a)Abstract / p17 (0031.jp2)
- (b)Introduction / p17 (0031.jp2)
- (c)Experimental Procedure / p18 (0032.jp2)
- (d)Results and Discussion / p21 (0035.jp2)
- References / p25 (0039.jp2)
- Chapter 3.Identification of the Secondary Structure of αCTD / p27 (0052.jp2)
- (a)Abstract / p28 (0053.jp2)
- (b)Introduction / p28 (0053.jp2)
- (c)Experimental Procedure / p29 (0054.jp2)
- (d)Results and Discussion / p30 (0055.jp2)
- References / p34 (0059.jp2)
- Chapter 4.Three-dimenaional Structure Determination of αCTD / p36 (0067.jp2)
- (a)Abstract / p37 (0068.jp2)
- (b)Introduction / p37 (0068.jp2)
- (c)Experimental Procedure / p37 (0068.jp2)
- (d)Results and Discussion / p38 (0069.jp2)
- References / p41 (0072.jp2)
- Chapter 5.Interactions of the αCTD with DNA and Transcription Activator Proteins / p43 (0080.jp2)
- (a)Abstract / p44 (0081.jp2)
- (b)Introduction / p44 (0081.jp2)
- (c)Experimental Procedure / p44 (0081.jp2)
- (d)Results and Discussion / p45 (0082.jp2)
- References / p50 (0087.jp2)
- Chapter 6.Molecular Dynamics of αCTD as an Isolated Domain and in the Intact Whole α Subunit / p53 (0096.jp2)
- (a)Abstract / p54 (0097.jp2)
- (b)Introduction / p54 (0097.jp2)
- (c)Experimental Procedure / p55 (0098.jp2)
- (d)Results and Discussion / p56 (0099.jp2)
- References / p60 (0103.jp2)
- Conclusion / p62 (0111.jp2)
