Studies on culture of rat 1-cell embryos in a chemically defined medium 限定培地におけるラット1-細胞期胚の培養に関する研究
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著者
書誌事項
- タイトル
-
Studies on culture of rat 1-cell embryos in a chemically defined medium
- タイトル別名
-
限定培地におけるラット1-細胞期胚の培養に関する研究
- 著者名
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三好, 和睦
- 著者別名
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ミヨシ, カズチカ
- 学位授与大学
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岡山大学
- 取得学位
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博士 (農学)
- 学位授与番号
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甲第1494号
- 学位授与年月日
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1996-03-25
注記・抄録
博士論文
目次
- CONTENTS / p3 (0005.jp2)
- PREFACE / p1 (0003.jp2)
- ACKNOWLEDGMENTS / p2 (0004.jp2)
- CONTENTS / p3 (0005.jp2)
- LIST OF TABLES / p6 (0008.jp2)
- LIST OF FIGURES / p8 (0010.jp2)
- ABSTRACT / p9 (0011.jp2)
- INTRODUCTION / p1 (0013.jp2)
- CHAPTER1.GENERAL METHODOLOGY / p4 (0016.jp2)
- 1.Media / p4 (0016.jp2)
- 2.Collection and culture of 1-cell embryos fertilized in vivo / p4 (0016.jp2)
- 3.Preparation of sperm suspension for in vitro fertilization / p5 (0017.jp2)
- 4.Collection of eggs and in vitro fertilization / p5 (0017.jp2)
- 5.Examination of eggs / p6 (0018.jp2)
- 6.Culture of penetrated eggs / p6 (0018.jp2)
- 7.Evaluation of embryo development / p6 (0018.jp2)
- 8.Count of cell number of blastocysts / p6 (0018.jp2)
- 9.Transfer of embryos developed in culture / p7 (0019.jp2)
- 10.Statistical analysis / p7 (0019.jp2)
- CHAPTER2.EFFECTS OF GLUCOSE,PHOSPHATE AND OSMOLARITY IN A CHEMICALLY DEFINED MEDIUM ON DEVELOPMENT OF RAT 1-CELL EMBRYOS / p8 (0020.jp2)
- Materials and Methods / p8 (0020.jp2)
- Results / p9 (0021.jp2)
- Discussion / p12 (0024.jp2)
- Summary / p18 (0030.jp2)
- CHAPTER3.EFFECTS OF OSMOLARITY AND AMINO ACIDS IN A CHEMICALLY DEFINED MEDIUM ON DEVELOPMENT OF RAT 1-CELL EMBRYOS / p19 (0031.jp2)
- Materials and Methods / p19 (0031.jp2)
- Results / p22 (0034.jp2)
- Discussion / p24 (0036.jp2)
- Summary / p29 (0041.jp2)
- CHAPTER4.PHOSPHATE REQUIREMENT FOR DEVELOPMENT OF RAT 1-CELL EMBRYOS IN A CHEMICALLY DEFINED MEDIUM / p31 (0043.jp2)
- Materials and Methods / p31 (0043.jp2)
- Results / p33 (0045.jp2)
- Discussion / p37 (0049.jp2)
- Summary / p42 (0054.jp2)
- CHAPTER5.DEVELOPMENT OF RAT 1-CELL EMBRYOS DERIVED FROM IN VITRO FERTILIZATION AND CULTURED IN A CHEMICALLY DEFINED MEDIUM,mR1ECM / p44 (0056.jp2)
- Materials and Methods / p44 (0056.jp2)
- Results / p45 (0057.jp2)
- Discussion / p47 (0059.jp2)
- Summary / p50 (0062.jp2)
- CHAPTER6.REQUIREMENTS OF SOME FACTORS FOR THE DEVELOPMENT IN DEFINED CONDITIONS OF RAT 1-CELL EMBRYOS SOON AFTER PENETRATION IN VIVO AND IN VITRO / p51 (0063.jp2)
- Materials and Methods / p51 (0063.jp2)
- Results / p53 (0065.jp2)
- Discussion / p56 (0068.jp2)
- Summary / p62 (0074.jp2)
- CONCLUSION / p64 (0076.jp2)
- REFERENCES / p68 (0080.jp2)
- ABSTRACT IN JAPANESE / p82 (0094.jp2)