Development of highly efficient and inducible mammalian expression systems 高効率及び誘導可能な動物細胞発現システムの開発
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著者
書誌事項
- タイトル
-
Development of highly efficient and inducible mammalian expression systems
- タイトル別名
-
高効率及び誘導可能な動物細胞発現システムの開発
- 著者名
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張, 英培
- 著者別名
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チョウ, エイバイ
- 学位授与大学
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九州大学
- 取得学位
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博士 (農学)
- 学位授与番号
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甲第4163号
- 学位授与年月日
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1997-09-22
注記・抄録
博士論文
目次
- Contents / p1 (0003.jp2)
- Chapter 1 Recombinant protein production in mammalian cells: problems and solutions / p1 (0008.jp2)
- 1-1 Introduction / p1 (0008.jp2)
- 1-2 Strategy for enhancing foreign gene expression in mammalian cells / p2 (0009.jp2)
- 1-3 Novel strategies to improve foreign gene expression in mammalian cells / p13 (0020.jp2)
- Chapter 2 Analysis of the signaling pathways regulating the activity of CMV promoter in CHO cells / p15 (0022.jp2)
- 2-1 Introduction / p15 (0022.jp2)
- 2-2 Materials and Methods / p17 (0024.jp2)
- 2-3 Results / p21 (0028.jp2)
- 2-4 Discussions / p23 (0030.jp2)
- 2-5 Conclusions / p24 (0031.jp2)
- Chapter 3 Evidence that phosphatidylcholine-specific phospholipase C is a key molecule mediating insulin-induced enhancement of human interleukin 6 expression driven by human cytomegalovirus promoter in CHO cells / p25 (0032.jp2)
- 3-1 Introduction / p25 (0032.jp2)
- 3-2 Materials and methods / p27 (0034.jp2)
- 3-3 Results / p29 (0036.jp2)
- 3-4 Discussions / p33 (0040.jp2)
- 3-5 Conclusions / p36 (0043.jp2)
- Chapter 4 Enhancement of cellular productivity for recombinant proteins by combination of promoter activation and gene amplification / p37 (0044.jp2)
- 4-1 Introduction / p37 (0044.jp2)
- 4-2 Materials and methods / p38 (0045.jp2)
- 4-3 Results / p40 (0047.jp2)
- 4-4 Conclusions / p42 (0049.jp2)
- Chapter 5 Efficient and inducible production of human interleukin 6 in Chinese hamster ovary cells using a novel expression system / p43 (0050.jp2)
- 5-1 Introduction / p43 (0050.jp2)
- 5-2 Materials and methods / p46 (0053.jp2)
- 5-3 Results / p49 (0056.jp2)
- 5-4 Discussion / p54 (0061.jp2)
- 5-5 Conclusion / p55 (0062.jp2)
- Chapter 6 An autocatalytic expression system for regulated production of recombinant proteins in mammalian cells / p56 (0063.jp2)
- 6-1 Introduction / p56 (0063.jp2)
- 6-2 Materials and methods / p58 (0065.jp2)
- 6-3 Results / p62 (0069.jp2)
- 6-4 Discussions / p71 (0078.jp2)
- 6-5 Conclusions / p74 (0081.jp2)
- Chapter 7 A regulatable selection system facilitating isolation of cells inducibly over-producing heterologous protein / p75 (0082.jp2)
- 7-1 Introduction / p75 (0082.jp2)
- 7-2 Materials and methods / p78 (0085.jp2)
- 7-3 Results / p83 (0090.jp2)
- 7-4 Discussions / p91 (0098.jp2)
- 7-5 Conclusions / p94 (0101.jp2)
- Chapter 8 Summary / p95 (0102.jp2)
- Acknowledgments / p97 (0104.jp2)
- References / p98 (0105.jp2)