Development of highly efficient and inducible mammalian expression systems 高効率及び誘導可能な動物細胞発現システムの開発

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著者

    • 張, 英培 チョウ, エイバイ

書誌事項

タイトル

Development of highly efficient and inducible mammalian expression systems

タイトル別名

高効率及び誘導可能な動物細胞発現システムの開発

著者名

張, 英培

著者別名

チョウ, エイバイ

学位授与大学

九州大学

取得学位

博士 (農学)

学位授与番号

甲第4163号

学位授与年月日

1997-09-22

注記・抄録

博士論文

目次

  1. Contents / p1 (0003.jp2)
  2. Chapter 1 Recombinant protein production in mammalian cells: problems and solutions / p1 (0008.jp2)
  3. 1-1 Introduction / p1 (0008.jp2)
  4. 1-2 Strategy for enhancing foreign gene expression in mammalian cells / p2 (0009.jp2)
  5. 1-3 Novel strategies to improve foreign gene expression in mammalian cells / p13 (0020.jp2)
  6. Chapter 2 Analysis of the signaling pathways regulating the activity of CMV promoter in CHO cells / p15 (0022.jp2)
  7. 2-1 Introduction / p15 (0022.jp2)
  8. 2-2 Materials and Methods / p17 (0024.jp2)
  9. 2-3 Results / p21 (0028.jp2)
  10. 2-4 Discussions / p23 (0030.jp2)
  11. 2-5 Conclusions / p24 (0031.jp2)
  12. Chapter 3 Evidence that phosphatidylcholine-specific phospholipase C is a key molecule mediating insulin-induced enhancement of human interleukin 6 expression driven by human cytomegalovirus promoter in CHO cells / p25 (0032.jp2)
  13. 3-1 Introduction / p25 (0032.jp2)
  14. 3-2 Materials and methods / p27 (0034.jp2)
  15. 3-3 Results / p29 (0036.jp2)
  16. 3-4 Discussions / p33 (0040.jp2)
  17. 3-5 Conclusions / p36 (0043.jp2)
  18. Chapter 4 Enhancement of cellular productivity for recombinant proteins by combination of promoter activation and gene amplification / p37 (0044.jp2)
  19. 4-1 Introduction / p37 (0044.jp2)
  20. 4-2 Materials and methods / p38 (0045.jp2)
  21. 4-3 Results / p40 (0047.jp2)
  22. 4-4 Conclusions / p42 (0049.jp2)
  23. Chapter 5 Efficient and inducible production of human interleukin 6 in Chinese hamster ovary cells using a novel expression system / p43 (0050.jp2)
  24. 5-1 Introduction / p43 (0050.jp2)
  25. 5-2 Materials and methods / p46 (0053.jp2)
  26. 5-3 Results / p49 (0056.jp2)
  27. 5-4 Discussion / p54 (0061.jp2)
  28. 5-5 Conclusion / p55 (0062.jp2)
  29. Chapter 6 An autocatalytic expression system for regulated production of recombinant proteins in mammalian cells / p56 (0063.jp2)
  30. 6-1 Introduction / p56 (0063.jp2)
  31. 6-2 Materials and methods / p58 (0065.jp2)
  32. 6-3 Results / p62 (0069.jp2)
  33. 6-4 Discussions / p71 (0078.jp2)
  34. 6-5 Conclusions / p74 (0081.jp2)
  35. Chapter 7 A regulatable selection system facilitating isolation of cells inducibly over-producing heterologous protein / p75 (0082.jp2)
  36. 7-1 Introduction / p75 (0082.jp2)
  37. 7-2 Materials and methods / p78 (0085.jp2)
  38. 7-3 Results / p83 (0090.jp2)
  39. 7-4 Discussions / p91 (0098.jp2)
  40. 7-5 Conclusions / p94 (0101.jp2)
  41. Chapter 8 Summary / p95 (0102.jp2)
  42. Acknowledgments / p97 (0104.jp2)
  43. References / p98 (0105.jp2)
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各種コード

  • NII論文ID(NAID)
    500000151786
  • NII著者ID(NRID)
    • 8000001068725
  • DOI(NDL)
  • NDL書誌ID
    • 000000316100
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション
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