Cold adaptation of heat stable enzyme, 3-isopropylmalate dehydrogenase from extreme thermophiles 高度好熱菌由来耐熱性3-イソプロピルリンゴ酸脱水素酵素の低温適応

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著者

    • 鈴木, 俊治 スズキ, トシハル

書誌事項

タイトル

Cold adaptation of heat stable enzyme, 3-isopropylmalate dehydrogenase from extreme thermophiles

タイトル別名

高度好熱菌由来耐熱性3-イソプロピルリンゴ酸脱水素酵素の低温適応

著者名

鈴木, 俊治

著者別名

スズキ, トシハル

学位授与大学

東京工業大学

取得学位

博士 (理学)

学位授与番号

甲第3474号

学位授与年月日

1997-03-26

注記・抄録

博士論文

目次

  1. 論文目録 / (0002.jp2)
  2. CONTENTS / p3 (0005.jp2)
  3. Acknowledgments / p2 (0004.jp2)
  4. Chapter1 / p5 (0007.jp2)
  5. General Introduction / p5 (0007.jp2)
  6. Molecular adaptation of enzymes / p5 (0007.jp2)
  7. Heat stable enzymes from thermophilic bacteria / p5 (0007.jp2)
  8. Do catalytic activity and heat stability of the structure correlate? / p6 (0008.jp2)
  9. Cold adapted mutant enzymes / p7 (0009.jp2)
  10. Random mutagenesis to make cold-adapted mutant enzymes / p7 (0009.jp2)
  11. Extreme thermophiles,Thermus thermophilus and Sulfolobus sp.strain7 / p8 (0010.jp2)
  12. 3-Isopropylmalate dehydrogenase / p8 (0010.jp2)
  13. Contents of this thesis / p9 (0011.jp2)
  14. Chapter2 / p16 (0018.jp2)
  15. Naturally occurring cold adaptation of enzyme;The Molecular Evolution of 3-Isopropylmalate Dehydrogenase / p16 (0018.jp2)
  16. Molecular and Phylogenetic Characterization of 3-Isopropylmalate Dehydrogenase from a Thermoacidophilic archaeon,Sulfolobus sp.strain7 / p16 (0018.jp2)
  17. Chapter3 / p36 (0038.jp2)
  18. Further Characterization of Heat Stable 3-Isopropylmalate Dehydrogenase of Sulfolobus sp.Strain7 / p36 (0038.jp2)
  19. Crystallization and Preliminary X-Ray Study of Heat Stable 3-Isopropylmalate Dehydrogenase of an Acidothermophiloc Archaeon,Sulfolobus sp.strain7 / p36 (0038.jp2)
  20. Chapter4 / p42 (0044.jp2)
  21. First trial:In vitro screening of mutant plasmid with the enhanced expression efficiency of Thermus thermophilus IPMDH / p42 (0044.jp2)
  22. Improvement of Expression Efficiency of GC-Rich leuB Gene of an Extreme Thermophile,Thermus thermophilus,in Escherichia coli / p42 (0044.jp2)
  23. Chapter5 / p52 (0054.jp2)
  24. Second trial:In vitro screening of cold adapted mutant IPMDHs on the chromosome of Escherichia coli / p52 (0054.jp2)
  25. Screening of Cold-adapted Mutants from a Thermostable Enzyme in Escherichia coli;Improvement of catalytic activity of an enzyme without anyloss of its thermostability / p52 (0054.jp2)
  26. Chapter6 / p72 (0074.jp2)
  27. Third trial:Brief method for isolating cold-adapted mutant enzymes / p72 (0074.jp2)
  28. Isolation and Characterization of Cold-Adapted Mutant of Thermophilic Enzyme with Enhanced Eatalytic Ectivity at Mesophilic Temperature by PCR-Random Mutagenesis / p72 (0074.jp2)
  29. Chapter7 / p82 (0084.jp2)
  30. Overview and Concluding Remarks / p82 (0084.jp2)
  31. (I)Molecular evolution of heat-stable enzymes / p82 (0084.jp2)
  32. (II)Isolation and characterization of cold-adapted mutant IPMDHs / p83 (0085.jp2)
  33. Appendix I / p95 (0097.jp2)
  34. Appendix II / p97 (0099.jp2)
  35. Naturally occurring cold adaptation of enzyme;The Molecular Evolution of Ferredoxin / p97 (0099.jp2)
  36. Novel Zinc-containing Ferredoxin Family in Thermoacidophilic Archaeon / p97 (0099.jp2)
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各種コード

  • NII論文ID(NAID)
    500000153676
  • NII著者ID(NRID)
    • 8000001092691
  • DOI(NDL)
  • NDL書誌ID
    • 000000317990
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション
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