The novel apoptosis factor identification and new model for apoptosis : cytochrome c and its relationship with Bcl-2 新しいアポトーシス因子の同定と新たなアポトーシス・モデル : シトクロムcとそのBcl-2との関連性

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著者

    • 楊, 杰 ヤン, ジェ

書誌事項

タイトル

The novel apoptosis factor identification and new model for apoptosis : cytochrome c and its relationship with Bcl-2

タイトル別名

新しいアポトーシス因子の同定と新たなアポトーシス・モデル : シトクロムcとそのBcl-2との関連性

著者名

楊, 杰

著者別名

ヤン, ジェ

学位授与大学

東北大学

取得学位

博士(医学)

学位授与番号

乙第7658号

学位授与年月日

1999-03-03

注記・抄録

博士論文

目次

  1. TABLE OF CONTENTS / p2 (0004.jp2)
  2. 1. ABSTRACT / p4 (0006.jp2)
  3. 2. INTRODUCTION / p6 (0008.jp2)
  4. 1) The morphological and biochemical features of apoptosis / p6 (0008.jp2)
  5. 2) Molecular mechanism of programmed cell death in C.elegans / p6 (0008.jp2)
  6. 3) Regulation of apoptosis by Bcl-2 family in mammalian cells / p7 (0009.jp2)
  7. 4) Regulation of apoptosis by other mechanisms in mammalian cells / p7 (0009.jp2)
  8. 5) Regulation of apoptosis by ICE family proteases (caspases) / p8 (0010.jp2)
  9. 6) The interests of this study / p9 (0011.jp2)
  10. 3. EXPERIMENTAL PROCEDURES / p11 (0013.jp2)
  11. 1) Preparation of S-100 from HeLa,293 and U937 cells / p11 (0013.jp2)
  12. 2) In vitro translation of CPP32 and PARP / p11 (0013.jp2)
  13. 3) Western blot analysis / p12 (0014.jp2)
  14. 4) Assay for dATP-dependent activation of CPP32 protease / p12 (0014.jp2)
  15. 5) Purification of Apaf-2 from S-100 of HeLa cells / p12 (0014.jp2)
  16. 6) Preparation of hamster liver nuclei / p13 (0015.jp2)
  17. 7) DNA fragmentation assay / p13 (0015.jp2)
  18. 8) Immunodepletion of cytochrome c from HeLa S-100 / p14 (0016.jp2)
  19. 9) Isolation of mitochondrial and cytochrome c release assay / p14 (0016.jp2)
  20. 10) Stable transfection of Bcl-2 into HL-60 cells / p14 (0016.jp2)
  21. 11) Preparation of apocytochrome c / p15 (0017.jp2)
  22. 4. RESULTS / p16 (0018.jp2)
  23. 1) dATP-dependent activation of CPP32 and DNA fragmentation / p16 (0018.jp2)
  24. 2) Fractionation of proteins required for dATP-dependent activation of CPP32 / p16 (0018.jp2)
  25. 3) Purification of Apaf-2 / p17 (0019.jp2)
  26. 4) Identification of 15-kDa Apaf-2 as cytochrome c / p17 (0019.jp2)
  27. 5) Immunodepletion of cytochrome c and reconstitution of the cytochrome c-dependent apoptotic program / p18 (0020.jp2)
  28. 6) Release of cytochrome c into the cytosol upon apoptotic stimulation / p18 (0020.jp2)
  29. 7) Induction of cytochrome c release from the mitochondria into the cytosol by staurosporine / p19 (0021.jp2)
  30. 8) Bcl-2 prevents apoptosis by blocking the release of cytochrome c / p20 (0022.jp2)
  31. 9) Mitochondrial membrane potential alteration later than cytochrome c release / p21 (0023.jp2)
  32. 10) Induction of cytochrome c release into the cytosol by etoposide / p21 (0023.jp2)
  33. 11) In vitro reconstitution of CPP32 activation reaction / p22 (0024.jp2)
  34. 12) Apocytochrome c fails to substitute cytochrome c in the activation of the apoptotic program / p23 (0025.jp2)
  35. 5. DISCUSSION / p24 (0026.jp2)
  36. 1) In vitro system of apoptosis / p24 (0026.jp2)
  37. 2) dATP/dADP as the signal for apoptosis / p24 (0026.jp2)
  38. 3) Cytochrome c as a required component of the cell suicide program / p25 (0027.jp2)
  39. 4) Bcl-2 functions in the upstream of the ICE family proteases by blocking the release of cytochrome c / p26 (0028.jp2)
  40. 6. CONCLUSION / p28 (0030.jp2)
  41. 7. ACKNOWLEDGMENTS / p29 (0031.jp2)
  42. 8. REFERENCES / p30 (0032.jp2)
  43. 9. TABLE / p35 (0037.jp2)
  44. 10. FIGURES AND FIGURE LEGENDS / p36 (0038.jp2)
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各種コード

  • NII論文ID(NAID)
    500000171254
  • NII著者ID(NRID)
    • 8000000171528
  • DOI(NDL)
  • NDL書誌ID
    • 000000335568
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
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