Regulatory mechanism of organization of golgi apparatus 哺乳動物細胞におけるゴルジ体の形態維持機構に関する研究

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Author

    • 山口, 智広 ヤマグチ, トモヒロ

Bibliographic Information

Title

Regulatory mechanism of organization of golgi apparatus

Other Title

哺乳動物細胞におけるゴルジ体の形態維持機構に関する研究

Author

山口, 智広

Author(Another name)

ヤマグチ, トモヒロ

University

九州大学

Types of degree

博士(薬学)

Grant ID

甲第4698号

Degree year

1999-03-25

Note and Description

博士論文

GENERAL INTRODUCTION PART I.INVOLVEMENT OF HETEROTRIMERIC G PROTEINS IN THE ORGANIZATION OF THE GOLGI APPARATUS PART II.ADP-RIBOSYLATION FACTOR I IS SENSITIVE TO N-ETHYLMALEIMIDE SUMMARY

Table of Contents

  1. CONTENTS / p1 (0003.jp2)
  2. GENERAL INTRODUCTION / p4 (0006.jp2)
  3. PART I.INVOLVEMENT OF HETEROTRIMERIC G PROTEINS IN THE ORGANIZATION OF THE GOLGI APPARATUS / p8 (0010.jp2)
  4. 1.INTRODUCTION / p8 (0010.jp2)
  5. 2.EXPERIMENTAL PROCEDURES / p10 (0012.jp2)
  6. 2.1 Materials / p10 (0012.jp2)
  7. 2.2 Cell Culture / p10 (0012.jp2)
  8. 2.3 Permeabilization and Incubation Conditions / p11 (0013.jp2)
  9. 2.4 Pertussis and Cholera Toxin Treatment / p11 (0013.jp2)
  10. 2.5 Immunofluorescence and Electron Microscopic Analysis / p11 (0013.jp2)
  11. 2.6 Expression Vectors and Transfection / p12 (0014.jp2)
  12. 3.RESULTS / p13 (0015.jp2)
  13. 3.1 NDGA Causes Disassembly of the Golgi Apparatus in a Microtubule-independent Manner / p13 (0015.jp2)
  14. 3.2 β-COP Is not Released by NDGA / p14 (0016.jp2)
  15. 3.3 AlF₄‾ or Mastoparan Markedly Prevents the Disassembly of the Golgi Apparatus / p14 (0016.jp2)
  16. 3.4 GTPγS Blocks the NDGA-promoted Disassembly of the Golgi Apparatus / p20 (0022.jp2)
  17. 3.5 Effect of βγ-Subunits of Heterotrimeric G proteins / p21 (0023.jp2)
  18. 3.6 Effect of Pertussis and Cholera Toxins / p24 (0026.jp2)
  19. 3.7 Overexpression of α-subunits of heterotrimeric G proteins / p24 (0026.jp2)
  20. 3.8 Overexpression of βγ-subunit of heterotrimeric G proteins / p25 (0027.jp2)
  21. 4.DISCUSSION / p32 (0034.jp2)
  22. PART II.ADP-RIBOSYLATION FACTOR I IS SENSITIVE TO N-ETHYLMALEIMIDE / p37 (0039.jp2)
  23. 1.INTRODUCTION / p37 (0039.jp2)
  24. 2.EXPERIMENTAL PROCEDURES / p38 (0040.jp2)
  25. 2.1 Materials / p38 (0040.jp2)
  26. 2.2 Cell Culture and Immunofluores cence Analysis / p38 (0040.jp2)
  27. 2.3 Permeabilization and Incubation Conditions / p38 (0040.jp2)
  28. 2.4 Preparation of ARF-rich and Coatomer-rich Fractions / p38 (0040.jp2)
  29. 2.5 Binding of Coatomer Proteins to Isolated Golgi Membranes / p39 (0041.jp2)
  30. 2.6 Determination of the Sulfhydryl Content / p39 (0041.jp2)
  31. 3.RESULTS AND DISCUSSION / p40 (0042.jp2)
  32. 3.1 β-COP is Released from the Golgi Apparatus by NEM-Treatment / p40 (0042.jp2)
  33. 3.2 Activation of Heterotrimeric G Proteins Markedly Prevents the NEM-Induced Release of β-COP from the Golgi Membranes / p41 (0043.jp2)
  34. 3.3 Cytosolic Proteins Are NEM-Sensitive / p41 (0043.jp2)
  35. 3.4 ARF Is Sensitive to NEM / p45 (0047.jp2)
  36. 3.5 Cys-159 Is the Only Cysteine Residue in ARF / p45 (0047.jp2)
  37. SUMMARY / p54 (0056.jp2)
  38. ACKNOWLEDGEMENTS / p56 (0058.jp2)
  39. REFERENCES / p57 (0059.jp2)
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Codes

  • NII Article ID (NAID)
    500000171674
  • NII Author ID (NRID)
    • 8000000171948
  • DOI(NDL)
  • Text Lang
    • eng
  • NDLBibID
    • 000000335988
  • Source
    • Institutional Repository
    • NDL ONLINE
    • NDL Digital Collections

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