Increased type 2 cytokine expression by both CD4[+] CD45RO[+] T cells and CD8[+] CD45RO[+] T cells in blood circulation is associated with high serum IgE but not with atopic dermatitis アトピー性皮膚炎患者の末梢血CD4[+]CD45RO[+]T細胞とCD8[+]CD45RO[+]T細胞におけるType2サイトカイン産生能と血清IgE値の関連

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著者

    • 佐藤, 淳 サトウ, アツシ

書誌事項

タイトル

Increased type 2 cytokine expression by both CD4[+] CD45RO[+] T cells and CD8[+] CD45RO[+] T cells in blood circulation is associated with high serum IgE but not with atopic dermatitis

タイトル別名

アトピー性皮膚炎患者の末梢血CD4[+]CD45RO[+]T細胞とCD8[+]CD45RO[+]T細胞におけるType2サイトカイン産生能と血清IgE値の関連

著者名

佐藤, 淳

著者別名

サトウ, アツシ

学位授与大学

岡山大学

取得学位

博士(医学)

学位授与番号

甲第1847号

学位授与年月日

1999-03-25

注記・抄録

博士論文

Type 2 cytokines, such as interleukin-4 (IL-4) and IL-13, are associated with immunoglobulin E (IgE) production. This association has also been observed in CD8+ T cells from patients infected with leprosy and human immunodeficiency virus (HIV). Using intracellular cytokine staining and flow cytometry, the cytokine profile [IL-2, IL-4, IL-10, IL-13, and interferon (IFN)-gamma] of both CD4+ and CD8+ memory/effector T cells circulating in atopic dermatitis (AD) patients was investigated at the single cell level. The levels of type 2 cytokines in CD4+ T cells or CD8+ T cells in AD patients with high levels of serum IgE (AD-H), low levels of serum IgE (AD-L), and healthy controls were compared. Increased production of IL-4 and IL-13 in both CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells after 4 h in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin, was more prominent in AD-H patients than in AD-L patients or healthy controls, whereas IFN-gamma-producing CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells were relatively diminished in AD-H patients. CD4+ T cells and CD8 + T cells from AD-H patients, cultured for 48 h with phorbol 12-myristate 13-acetate and ionomycin, released larger amounts of IL-4 and IL-13 but smaller amounts of IFN-gamma than both types of cells from AD-L patients or healthy controls. In addition, when stimulated with immobilized anti-CD3 monoclonal antibody (MoAb) and anti-CD28 MoAb, CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells from AD-H patients contained more IL-4-producing cells but fewer IFN-gamma-producing cells compared with healthy controls. Finally, spontaneous mRNA expression of IL-4 in blood CD8+ CD45RO+ T cells isolated from AD-H patients was increased, as determined by reverse transcriptase-polymerase chain reaction. Therefore, in AD patients with high IgE levels, type 2 cytokine (IL-4 and IL-13) expression is associated with IgE production, in both CD4+ CD45RO+ T cell and CD8+ CD45RO+ T cell subsets.

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各種コード

  • NII論文ID(NAID)
    500000174229
  • NII著者ID(NRID)
    • 8000000174505
  • DOI(NDL)
  • 本文言語コード
    • jpn
  • NDL書誌ID
    • 000000338543
  • データ提供元
    • 機関リポジトリ
    • NDL-OPAC
    • NDLデジタルコレクション
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