Studies on the developmental block induced by phosphate in 1-cell rat embryos cultured in a chemically defined medium

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タイトル: Studies on the developmental block induced by phosphate in 1-cell rat embryos cultured in a chemically defined medium

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PREFACE / p1 (0003.jp2)
ACKNOWLEDGMENTS / p2 (0004.jp2)
CONTENTS / p3 (0005.jp2)
LIST OF TABLES / p5 (0007.jp2)
LIST OF FIGURES / p6 (0008.jp2)
ABSTRACT / p7 (0009.jp2)
INTRODUCTION / p1 (0010.jp2)
CHAPTER1.GENERAL METHODOLOGY / p4 (0013.jp2)
1.Media / p4 (0013.jp2)
2.Collection and culture of 1-cell embryos fertilized in vivo / p4 (0013.jp2)
3.Preparation of sperm suspension for in vitro fertilization / p5 (0014.jp2)
4.Collection of ovulated eggs and in vitro fertilization / p6 (0015.jp2)
5.Assessment of sperm penetration / p6 (0015.jp2)
6.Culture of embryos fertilized in vitro and assessment of embryonic development / p6 (0015.jp2)
7.Transfer of morulae and blastocyst to recipients / p7 (0016.jp2)
8.Measurement of DNA content / p7 (0016.jp2)
9.Staining and observation of actin microfilaments / p8 (0017.jp2)
10.Statistical analysis / p8 (0017.jp2)
CHAPTER2.EFFECTS OF PHOSPHATE DURING THE 2-CELL STAGE OF RAT EMBRYOS ON THEIR FURTHER DEVELOPMENT IN VITRO AND MICROFILAMENT ORGANIZATION / p9 (0018.jp2)
Materials and Methods / p10 (0019.jp2)
Results / p13 (0022.jp2)
Discussion / p18 (0027.jp2)
Summary / p25 (0034.jp2)
CHAPTER3.FERTILIZATION IN VITRO OF RAT OOCYTES IN MODIFIED RAT 1-CELL EMBRYO CULTURE MEDIUM(mR1ECM)AFFECTED BY SODIUM CHLORIDE CONCENTRATION AND BOVINE SERUM ALBUMIN / p27 (0036.jp2)
Materials and Methods / p28 (0037.jp2)
Results / p31 (0040.jp2)
Discussion / p34 (0043.jp2)
Summary / p38 (0047.jp2)
CONCLUSION / p40 (0049.jp2)
REFERENCES / p42 (0051.jp2)
ABSTRACT IN JAPANESE / p47 (0056.jp2)

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Studies on the developmental block induced by phosphate in 1-cell rat embryos cultured in a chemically defined medium 限定培地においてリン酸により誘起されるラット1-細胞期胚の発生停止に関する研究