Crystallographic studies of transcription factors bound to DNA : IRF-2/DNA and Pap1/DNA complexes 転写因子/DNA複合体における結晶学的研究 : IRF-2/DNA複合体とPap1/DNA複合体

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Author

    • 藤井, 佳史 フジイ, ヨシフミ

Bibliographic Information

Title

Crystallographic studies of transcription factors bound to DNA : IRF-2/DNA and Pap1/DNA complexes

Other Title

転写因子/DNA複合体における結晶学的研究 : IRF-2/DNA複合体とPap1/DNA複合体

Author

藤井, 佳史

Author(Another name)

フジイ, ヨシフミ

University

奈良先端科学技術大学院大学

Types of degree

博士(バイオサイエンス)

Grant ID

甲第70号

Degree year

1999-03-24

Note and Description

博士論文

Table of Contents

  1. 博士論文要旨 / (0003.jp2)
  2. Contents / p2 (0004.jp2)
  3. IRF-2/DNA complex / p4 (0006.jp2)
  4. 1 IRF-2/DNA Summary / p4 (0006.jp2)
  5. 2 Introduction / p5 (0007.jp2)
  6. 3 The background of X-ray crystallography / p10 (0012.jp2)
  7. ･The solution of the phase problem / p10 (0012.jp2)
  8. ･Isomorphous replacement method / p11 (0013.jp2)
  9. ･Patterson function / p11 (0013.jp2)
  10. ･Isomorphous replacement part2 / p13 (0015.jp2)
  11. ･Molecular replacement method / p14 (0016.jp2)
  12. ･The rotation function / p15 (0017.jp2)
  13. ･The translation function / p17 (0019.jp2)
  14. ･Estimation of the phase in isomorphous replacement method / p18 (0020.jp2)
  15. ･Refinement / p19 (0021.jp2)
  16. ･The Ramachandran plot / p20 (0022.jp2)
  17. 4 Materials and methods / p21 (0023.jp2)
  18. 4-1 Expression and purification / p21 (0023.jp2)
  19. 4-2 DNA synthesis and purification / p22 (0024.jp2)
  20. 4-3 Gel mobility shift assay / p24 (0026.jp2)
  21. 4-4 Crystallization / p24 (0026.jp2)
  22. 4-5 Data collection / p26 (0028.jp2)
  23. 4-6 Structure determination and refinement / p31 (0033.jp2)
  24. 4-7 Structure inspection and model building / p33 (0035.jp2)
  25. 5 Results / p34 (0036.jp2)
  26. 5-1 Overall structure / p34 (0036.jp2)
  27. 5-2 Characteristic tryptophan repeats and its hydrophobic contacts / p44 (0046.jp2)
  28. 5-3 DNA binding mechanism / p44 (0046.jp2)
  29. 5-4 DNA structure deformation / p49 (0051.jp2)
  30. 5-5 Metal binding site / p52 (0054.jp2)
  31. 5-6 The 5'-AA sequence indispensable binding / p54 (0056.jp2)
  32. 6 Discussion / p56 (0058.jp2)
  33. 6-1 Cooperative binding / p56 (0058.jp2)
  34. 6-2 IRF recognition sequence(IRS) / p58 (0060.jp2)
  35. 6-3 Implication of synergistic binding with other transcription factors / p61 (0063.jp2)
  36. IFN-β enhancesome / p61 (0063.jp2)
  37. VCAM-1 gene promoter / p64 (0066.jp2)
  38. 7 Conclusion / p66 (0068.jp2)
  39. Pap1/DNA complex / p67 (0069.jp2)
  40. 8 Pap1/DNA Summary / p67 (0069.jp2)
  41. 9 Introduction / p68 (0070.jp2)
  42. 10 Materials and methods / p72 (0074.jp2)
  43. 10-1 Expression / p72 (0074.jp2)
  44. 10-2 Purification / p72 (0074.jp2)
  45. 10-3 DNA synthesis and purification / p73 (0075.jp2)
  46. 10-4 Crystallization / p73 (0075.jp2)
  47. 10-5 Data collection / p74 (0076.jp2)
  48. 10-6 Structure determination and refinement / p74 (0076.jp2)
  49. 11 Results / p78 (0080.jp2)
  50. 11-1 Overall structure / p78 (0080.jp2)
  51. 11-2 DNA recognition / p82 (0084.jp2)
  52. 11-3 DNA deformation / p85 (0087.jp2)
  53. 12 Discussion / p88 (0090.jp2)
  54. 12-1 The specificity of nom-consensus Pap-1 target site / p88 (0090.jp2)
  55. 12-2 Parallel coiled-coil packing / p92 (0094.jp2)
  56. 13 Conclusion / p98 (0100.jp2)
  57. 14 Acknowledgments / p99 (0101.jp2)
  58. 15 References / p100 (0102.jp2)
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Codes

  • NII Article ID (NAID)
    500000174727
  • NII Author ID (NRID)
    • 8000000175004
  • DOI(NDL)
  • Text Lang
    • eng
  • NDLBibID
    • 000000339041
  • Source
    • Institutional Repository
    • NDL ONLINE
    • NDL Digital Collections

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