Changes and movements of the locus of amplified c-myc genes in HL-60 cells during culture or differentiation induction HL-60細胞の培養あるいは分化誘導に伴うc-myc遺伝子の増幅部位の変化と移動

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著者

    • Md. Masudul Haque モハメド マスドル ハク

書誌事項

タイトル

Changes and movements of the locus of amplified c-myc genes in HL-60 cells during culture or differentiation induction

タイトル別名

HL-60細胞の培養あるいは分化誘導に伴うc-myc遺伝子の増幅部位の変化と移動

著者名

Md. Masudul Haque

著者別名

モハメド マスドル ハク

学位授与大学

広島大学

取得学位

博士 (学術)

学位授与番号

甲第1938号

学位授与年月日

1999-03-08

注記・抄録

博士論文

目次

  1. CONTENTS / p1 (0003.jp2)
  2. ABBREVIATIONS / p6 (0008.jp2)
  3. SUMMARY OF MAJOR FINDINGS / p1 (0009.jp2)
  4. 1.Introduction / p4 (0012.jp2)
  5. 1.1 The human myeloblastic leukemia cell line HL-60 / p4 (0012.jp2)
  6. 1.2 Requirements of double minutes exclusion by micronucleation for spontaneous differentiation of HL-60 cells / p8 (0016.jp2)
  7. 1.3 Requirements of double minutes exclusion by micronucleation for drug-induced differentiation of HL-60 cells / p11 (0019.jp2)
  8. 1.4 Identical number of c-myc genes retained in HL-60 cells differentiated either spontaneously or by the drug treatment / p13 (0021.jp2)
  9. 1.5 Acceleration of growth, Dmins loss, and a decrease in the proportion of spontaneously differentiated cells by passaging HL-60 cells in vitro / p14 (0022.jp2)
  10. 1.6 Scope of this study / p16 (0024.jp2)
  11. 2.Experimental Procedures / p18 (0026.jp2)
  12. 2.1 Cell culture and drug treatment / p18 (0026.jp2)
  13. 2.2 Slide preparation for fluorescence in situ hybridization / p21 (0029.jp2)
  14. 2.3 Fluorescence in situ hybridization / p22 (0030.jp2)
  15. 2.4 Chromosome G-banding / p25 (0033.jp2)
  16. 3.Retention of Both of the Native c-myc Genes at the Original Chromosomal Sites in HL-60 Cells Independent of Movements of the Locus of Amplified c-myc Genes by Passage or Differentiation / p26 (0034.jp2)
  17. 3.1 HL-60 cells at various stages of passage / p26 (0034.jp2)
  18. 3.2 Differentiated HL-60 cells / p28 (0037.jp2)
  19. 4.Replacement of Double Minutes by Abnormally Staining Tiny Chromosomes in the Locus of Amplified c-myc Genes During Culture in Vitro / p32 (0044.jp2)
  20. 4.1 Presence of abnormally staining tiny chromosomes in a small population of cells in the original cell line and replacement of double minutes by passaging / p32 (0044.jp2)
  21. 4.2 No exclusion of abnormally staining tiny chromosomes by micronucleation / p37 (0051.jp2)
  22. 4.3 Reversible DMSO-induced differentiation in HL-60 cells harboring abnormally staining tiny chromosomes / p38 (0052.jp2)
  23. 4.4 Characterization of abnormally staining tiny chromosome / p40 (0055.jp2)
  24. 5.Localization of Retained c-myc Genes in Double Minutes in Differentiated HL-60 Cells at Low Passages / p44 (0061.jp2)
  25. 5.1 Localization of Retained c-myc genes in Dmins / p44 (0061.jp2)
  26. 5.2 Dmins quantification by a new method and verification of absence of correlation between c-myc gene exclusion and differentiation induction in low passage HL-60 cells / p44 (0061.jp2)
  27. 6.Finding of Dmins in The Cytoplasm of HL-60 Cells Harboring Dmins in The Cell Nuclei / p48 (0066.jp2)
  28. 6.1 Finding of Dmin-like structures(DMLSs)in the cytoplasm of HL-60 cells at early passages / p48 (0066.jp2)
  29. 6.2 Incorporation of 5-bromodeoxyuridine into DNA of cytoplasmic Dmins / p50 (0069.jp2)
  30. 7.Discussion / p51 (0070.jp2)
  31. 7.1 Molecular events that preserved the native c-myc genes throughout during initiation of c-myc gene amplification,ongoing transitions of the locus of amplified genes by passaging in vitro and differentiation induction / p51 (0070.jp2)
  32. 7.2 Changes and movements of the locus of amplified c-myc genes during passaging and differentiation induction / p54 (0073.jp2)
  33. 7.3 Properties of abnormally staining tiny chromosomes / p57 (0076.jp2)
  34. 7.4 Differentiation induction of HL-60 cells harboring abnormally staining tiny chromosomes / p58 (0077.jp2)
  35. 7.5 Gene(s)involved in inhibition of irreversible differentiation in HL-60 cells / p60 (0079.jp2)
  36. 7.6 Mechanisms by which Dmins appeared in the cytoplasm of HL-60 cells that harbor Dmins in nuclei / p63 (0082.jp2)
  37. 7.7 Concluding remarks / p64 (0083.jp2)
  38. ACKNOWLEDGEMENTS / p65 (0084.jp2)
  39. REFERENCES / p67 (0086.jp2)
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各種コード

  • NII論文ID(NAID)
    500000185195
  • NII著者ID(NRID)
    • 8000000185477
  • DOI(NDL)
  • NDL書誌ID
    • 000000349509
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション
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