Morphological and molecular biological analysis of sex chromosomes in hemp (Cannabis sativa L.) アサの性染色体の形態学的・分子生物学的解析
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Bibliographic Information
- Title
-
Morphological and molecular biological analysis of sex chromosomes in hemp (Cannabis sativa L.)
- Other Title
-
アサの性染色体の形態学的・分子生物学的解析
- Author
-
阪本, 浩一
- Author(Another name)
-
サカモト, コウイチ
- University
-
筑波大学
- Types of degree
-
博士 (理学)
- Grant ID
-
甲第2009号
- Degree year
-
1999-03-25
Note and Description
博士論文
1998
【要旨】
Table of Contents
- Table of Contents / p1 (0003.jp2)
- Abbreviations / p3 (0005.jp2)
- Abstract / p1 (0006.jp2)
- Introduction / p4 (0009.jp2)
- Materials and Methods / p10 (0015.jp2)
- Plant materials / p11 (0016.jp2)
- Preparation of the chromosomes / p11 (0016.jp2)
- Estimation of chromosome length / p12 (0017.jp2)
- Preparation of meiotic chromosomes / p12 (0017.jp2)
- Estimation of nuclear genome size / p13 (0018.jp2)
- Preparation of DNA / p14 (0019.jp2)
- Polymerase chain reaction(PCR) / p15 (0020.jp2)
- Gel electrophoresis and recovery of DNA fragments / p15 (0020.jp2)
- Preparation of DNA probes / p16 (0021.jp2)
- Gel blot analysis of genomic DNA / p16 (0021.jp2)
- Fluorescence in situ hybridization(FISH) / p16 (0021.jp2)
- Sequence analysis of DNA fragments / p17 (0022.jp2)
- Thermal asymmetric interlaced(TAIL) PCR / p18 (0023.jp2)
- Results / p19 (0024.jp2)
- Morphological characterization of sex chromosomes and determination of genome size / p20 (0025.jp2)
- Analysis of karyotype / p20 (0025.jp2)
- Dynamics of Y chromosome / p20 (0025.jp2)
- Analysis of meiotic chromosomes / p21 (0026.jp2)
- Estimation of nuclear genome size / p21 (0026.jp2)
- Cloning and characterization of male-associated DNA fragments / p22 (0027.jp2)
- PCR with arbitrary primers / p22 (0027.jp2)
- Gel blot analysis of genomic DNA / p23 (0028.jp2)
- Analysis of sequences / p24 (0029.jp2)
- Characterization of Y chromosome-associated sequence / p25 (0030.jp2)
- Fluorescence in situ hybridization(FISH) with MADC1 / p25 (0030.jp2)
- Cloning and characterization of retrotransposon / p25 (0030.jp2)
- Discussion / p28 (0033.jp2)
- References / p37 (0042.jp2)
- Table and Figures / p45 (0050.jp2)
- Acknowledgements / p83 (0087.jp2)