Molecular analysis of a MAP kinase cascade involved in environment stress response in Arabidopsis thaliana 環境ストレス応答におけるシロイヌナズナMAPキナーゼカスケードに関する分子生物学的解析

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著者

    • 市村, 和也 イチムラ, カズヤ

書誌事項

タイトル

Molecular analysis of a MAP kinase cascade involved in environment stress response in Arabidopsis thaliana

タイトル別名

環境ストレス応答におけるシロイヌナズナMAPキナーゼカスケードに関する分子生物学的解析

著者名

市村, 和也

著者別名

イチムラ, カズヤ

学位授与大学

筑波大学

取得学位

博士 (理学)

学位授与番号

甲第2016号

学位授与年月日

1999-03-25

注記・抄録

博士論文

1998

【要旨】

目次

  1. Contents / p1 (0003.jp2)
  2. Abbreviations / p4 (0006.jp2)
  3. Abstract / p1 (0008.jp2)
  4. Introduction / p8 (0015.jp2)
  5. Materials and Methods / p18 (0025.jp2)
  6. Plant materials / p19 (0026.jp2)
  7. Strains,media and yeast transformation / p19 (0026.jp2)
  8. Molecular biological techniques / p19 (0026.jp2)
  9. Two-hybrid screening / p20 (0027.jp2)
  10. Molecular cloning of a full-length cDNA for ATMKK2 / p21 (0028.jp2)
  11. Preparation of cDNA fragments for ATMKK3,4 and 5 for cDNA library screening / p21 (0028.jp2)
  12. Cloning and sequencing of cDNAs for ATMKK3,4 and 5 / p22 (0029.jp2)
  13. Southern and Northern blot analysis / p23 (0030.jp2)
  14. Expression of GST-fusion proteins in E. coli and phosphorylation assay of GST-fusion proteins / p23 (0030.jp2)
  15. Mapping of the ATMKK2,3,4 and 5 genes / p24 (0031.jp2)
  16. Cloning of ANP4 / p24 (0031.jp2)
  17. Two-hybrid and β-galactosidase assays / p25 (0032.jp2)
  18. Complementation of the growth defect of the pbs2Δ mutant by coexpression of Arabidopsis MAPKKs and MAPKKKs / p25 (0032.jp2)
  19. Complementation of the growth defects of the mpk1Δ and the bck1Δ mutants by coexpression of Arabidopsis MAPKs and MAPKKs / p26 (0033.jp2)
  20. Stress and phytohormone treatment / p26 (0033.jp2)
  21. Preparation of protein extracts / p27 (0034.jp2)
  22. Protein kinase assay / p27 (0034.jp2)
  23. Immunoprecipitation / p28 (0035.jp2)
  24. Antibody production and immunoblot analysis / p28 (0035.jp2)
  25. Results / p30 (0037.jp2)
  26. Part1:Isolation of ATMEKK1-interacting proteins using a yeast two-hybrid system / p31 (0038.jp2)
  27. Isolation of ATMEKK1-interacting proteins using a yeast two-hybrid system / p31 (0038.jp2)
  28. Nucleotide and amino acid sequence,and expression of ATMKK2(a MAPKK) / p33 (0040.jp2)
  29. Part2:Isolation and characterization of Arabidopsis MAPKK homologues / p34 (0041.jp2)
  30. Structural analysis of the putative ATMKK2,3,4 and 5 proteins / p35 (0042.jp2)
  31. Detection of protein kinase activity of the recombinant ATMKK3 and 4 proteins / p37 (0044.jp2)
  32. Northern and Southern blot analysis of the ATMKK3,4,and 5 genes / p38 (0045.jp2)
  33. Map positions of the ATMKK2,3,4 and 5 genes on Arabidopsis chromosomes / p39 (0046.jp2)
  34. Part3:Identification of a possible MAPK cascade based on yeast two-hybrid analysis and complementation of yeast mutants / p40 (0047.jp2)
  35. Analysis of complex formation between Arabidopsis MAPKKKs and MAPKKs using the yeast two-hybrid system / p40 (0047.jp2)
  36. Analysis of complex formation between Arabidopsis MAPKKs and MAPKs using the yeast two-hybrid system / p41 (0048.jp2)
  37. Analysis of complex formation between Arabidopsis MAPKs and MAPKKKs using the yeast two-hybrid system / p42 (0049.jp2)
  38. Deletion mappings of ATMPK4(a MAPK) and MEK1/ATMKK2(MAPKKs)interacting domains of ATMEKK1(a MAPKKK) / p43 (0050.jp2)
  39. Expression of ATMEKK1(a MAPKKK)increases activity of two closely related MAPKKs,MEK1 and ATMKK2,to complement the pbs2Δ defect / p44 (0051.jp2)
  40. Substitution of either threonine residue 220 or 226 with alanine completely abolishes the ability of A TMKK2 to suppress the pbs2 mutant / p45 (0052.jp2)
  41. Coexpression of MEK1(a MAPKK)and ATMPK4(a MAPK)complements the bck1Δ and mpk1Δ defects / p46 (0053.jp2)
  42. Part4: Effect of environmental stresses on the activation of Arabidopsis MAP kinase,ATMPK4 / p49 (0056.jp2)
  43. The ATMPK4CT antibody specifically recognize the ATMPK4 protein / p49 (0056.jp2)
  44. ATMPK4 is transiently activated by cold,dehumidification,touch and wounding stress treatments / p50 (0057.jp2)
  45. ATMPK4 is not activated by heat shock and ABA,partially activated by MeJA / p52 (0059.jp2)
  46. Low temperature but not osmotic shock,heat shock or ABA,induced activation of ATMPK4 in T87 cultured cell / p52 (0059.jp2)
  47. Expression of the ATMPK4 gene did not correlate with activity of ATMPK4 protein under stress conditions / p54 (0061.jp2)
  48. Protein level of ATMPK4 does not co-relate with its activity under stress conditions / p55 (0062.jp2)
  49. Discussion / p56 (0063.jp2)
  50. Isolation of ATMEKK1 binding proteins using yeast two-hybrid system / p57 (0064.jp2)
  51. Physical interactions among ATMEKK1(MAPKKK),MEK1/ATMKK2(MAPKKs) and ATMPK4(MAPK) / p58 (0065.jp2)
  52. Functional interactions among ATMEKK1(MAPKKK),MEK1/ATMKK2(MAPKKs) and ATMPK4(MAPK) / p61 (0068.jp2)
  53. ATMPK4,an Arabidopsis MAPK homologue,is activated by cold,dehumidification,touch and wounding stresses / p62 (0069.jp2)
  54. Possible roles of ATMPK4 kinase under cold and drought stress conditions / p63 (0070.jp2)
  55. A possible role of ATMPK4 under mechanical stress con ditions / p64 (0071.jp2)
  56. A possible role of ATMPK4 under wounding stress conditions / p65 (0072.jp2)
  57. Relationships between ATMPK4 and stress-activated MAPKs in plants / p66 (0073.jp2)
  58. A possible function of stress-activated ATMPK4-MAPK cascade in stress response / p68 (0075.jp2)
  59. Conclusions and perspectives / p71 (0078.jp2)
  60. Figures and Tables / p74 (0081.jp2)
  61. References / p123 (0130.jp2)
  62. Acknowledgements / p147 (0154.jp2)
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  • NII論文ID(NAID)
    500000185522
  • NII著者ID(NRID)
    • 8000000185804
  • DOI(NDL)
  • 本文言語コード
    • jpn
  • NDL書誌ID
    • 000000349836
  • データ提供元
    • 機関リポジトリ
    • NDL ONLINE
    • NDLデジタルコレクション

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