Studies on the physiological function of diacylglycerol and phosphatidic acid in dehydration stress response in Arabidopsis thaliana シロイヌナズナの乾燥ストレス応答におけるジアシルグリセロールとホスファチジン酸の生理機能の研究
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著者
書誌事項
- タイトル
-
Studies on the physiological function of diacylglycerol and phosphatidic acid in dehydration stress response in Arabidopsis thaliana
- タイトル別名
-
シロイヌナズナの乾燥ストレス応答におけるジアシルグリセロールとホスファチジン酸の生理機能の研究
- 著者名
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片桐, 健
- 著者別名
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カタギリ, タケシ
- 学位授与大学
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筑波大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第2017号
- 学位授与年月日
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1999-03-25
注記・抄録
博士論文
1998
【要旨】
目次
- Table of Contents / p1 (0003.jp2)
- Contents / p1 (0003.jp2)
- Abbreviations / p3 (0005.jp2)
- Abstract / p1 (0007.jp2)
- Introduction / p5 (0011.jp2)
- Materials and Methods / p10 (0016.jp2)
- Plant growth and stress treatment / p11 (0017.jp2)
- Yeast strains and growth conditions / p11 (0017.jp2)
- DAG measurement assay / p12 (0018.jp2)
- Polymerase chain reaction (PCR), isolation and sequencing of a cDNAs from Arabidopsis thaliana / p13 (0019.jp2)
- Northern blot analysis / p15 (0021.jp2)
- PAP assay / p16 (0022.jp2)
- PAP complementation using yeast system / p16 (0022.jp2)
- Generation of transgenic Arabidopsis / p17 (0023.jp2)
- Histochemical staining / p17 (0023.jp2)
- GUS activity measurement / p18 (0024.jp2)
- Western blot analysis / p18 (0024.jp2)
- Results / p19 (0025.jp2)
- Part I. Rapid and transient DAG induction by dehydration stress treatment / p20 (0026.jp2)
- Part II. Isolation and sequencing of two cDNAs that encode DGK from Arabidopsis thaliana / p21 (0027.jp2)
- Primary structure of the putative AtDGK1 and AtDGK2 protein / p22 (0028.jp2)
- Northern blot analysis of AtDGKs / p23 (0029.jp2)
- Part III. Cloning and sequence analysis of the AtPAP1 cDNA / p24 (0030.jp2)
- Primary structure of putative AtPAP1 / p24 (0030.jp2)
- PAP enzyme activity of the expressed AtPAP1 protein in Escherichia coli / p25 (0031.jp2)
- Genomic Southern blot analysis / p25 (0031.jp2)
- Northern blot analysis of AtPAP1 / p26 (0032.jp2)
- Transformation of AtPAP1 partially suppressed the abnormal growth of the yeast PAP null mutant / p26 (0032.jp2)
- Histochemical analysis of the GUS expression in the AtPAP1 promoter GUS transgenic plants / p27 (0033.jp2)
- Generation of the antisense AtPAP1 transgenic plant / p28 (0034.jp2)
- Western blot analysis of the antisense AtPAP1 transgenic plant / p29 (0035.jp2)
- Transient accumulation of DAG in response to dehydration was repressed in the antisense AtPAP1 transgenic plants / p29 (0035.jp2)
- Discussion / p31 (0037.jp2)
- Conclusion / p40 (0046.jp2)
- Figures and Table / p42 (0048.jp2)
- References / p74 (0080.jp2)
- Acknowledgments / p85 (0091.jp2)
