Studies on the physiological function of diacylglycerol and phosphatidic acid in dehydration stress response in Arabidopsis thaliana シロイヌナズナの乾燥ストレス応答におけるジアシルグリセロールとホスファチジン酸の生理機能の研究

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著者

    • 片桐, 健 カタギリ, タケシ

書誌事項

タイトル

Studies on the physiological function of diacylglycerol and phosphatidic acid in dehydration stress response in Arabidopsis thaliana

タイトル別名

シロイヌナズナの乾燥ストレス応答におけるジアシルグリセロールとホスファチジン酸の生理機能の研究

著者名

片桐, 健

著者別名

カタギリ, タケシ

学位授与大学

筑波大学

取得学位

博士 (理学)

学位授与番号

甲第2017号

学位授与年月日

1999-03-25

注記・抄録

博士論文

目次

  1. Table of Contents / p1 (0003.jp2)
  2. Contents / p1 (0003.jp2)
  3. Abbreviations / p3 (0005.jp2)
  4. Abstract / p1 (0007.jp2)
  5. Introduction / p5 (0011.jp2)
  6. Materials and Methods / p10 (0016.jp2)
  7. Plant growth and stress treatment / p11 (0017.jp2)
  8. Yeast strains and growth conditions / p11 (0017.jp2)
  9. DAG measurement assay / p12 (0018.jp2)
  10. Polymerase chain reaction (PCR), isolation and sequencing of a cDNAs from Arabidopsis thaliana / p13 (0019.jp2)
  11. Northern blot analysis / p15 (0021.jp2)
  12. PAP assay / p16 (0022.jp2)
  13. PAP complementation using yeast system / p16 (0022.jp2)
  14. Generation of transgenic Arabidopsis / p17 (0023.jp2)
  15. Histochemical staining / p17 (0023.jp2)
  16. GUS activity measurement / p18 (0024.jp2)
  17. Western blot analysis / p18 (0024.jp2)
  18. Results / p19 (0025.jp2)
  19. Part I. Rapid and transient DAG induction by dehydration stress treatment / p20 (0026.jp2)
  20. Part II. Isolation and sequencing of two cDNAs that encode DGK from Arabidopsis thaliana / p21 (0027.jp2)
  21. Primary structure of the putative AtDGK1 and AtDGK2 protein / p22 (0028.jp2)
  22. Northern blot analysis of AtDGKs / p23 (0029.jp2)
  23. Part III. Cloning and sequence analysis of the AtPAP1 cDNA / p24 (0030.jp2)
  24. Primary structure of putative AtPAP1 / p24 (0030.jp2)
  25. PAP enzyme activity of the expressed AtPAP1 protein in Escherichia coli / p25 (0031.jp2)
  26. Genomic Southern blot analysis / p25 (0031.jp2)
  27. Northern blot analysis of AtPAP1 / p26 (0032.jp2)
  28. Transformation of AtPAP1 partially suppressed the abnormal growth of the yeast PAP null mutant / p26 (0032.jp2)
  29. Histochemical analysis of the GUS expression in the AtPAP1 promoter GUS transgenic plants / p27 (0033.jp2)
  30. Generation of the antisense AtPAP1 transgenic plant / p28 (0034.jp2)
  31. Western blot analysis of the antisense AtPAP1 transgenic plant / p29 (0035.jp2)
  32. Transient accumulation of DAG in response to dehydration was repressed in the antisense AtPAP1 transgenic plants / p29 (0035.jp2)
  33. Discussion / p31 (0037.jp2)
  34. Conclusion / p40 (0046.jp2)
  35. Figures and Table / p42 (0048.jp2)
  36. References / p74 (0080.jp2)
  37. Acknowledgments / p85 (0091.jp2)
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各種コード

  • NII論文ID(NAID)
    500000185523
  • NII著者ID(NRID)
    • 8000000185805
  • DOI(NDL)
  • NDL書誌ID
    • 000000349837
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション
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