Studies on RING finger peroxins, Pex10p and Pex12p : cDNA Cloning and Functional Analysis RINGフィンガーペルオキシソーム形成因子,Pex10pおよびPex12pの構造と機能に関する研究
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著者
書誌事項
- タイトル
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Studies on RING finger peroxins, Pex10p and Pex12p : cDNA Cloning and Functional Analysis
- タイトル別名
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RINGフィンガーペルオキシソーム形成因子,Pex10pおよびPex12pの構造と機能に関する研究
- 著者名
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奥本, 寛治
- 著者別名
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オクモト, カンジ
- 学位授与大学
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九州大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第5006号
- 学位授与年月日
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2000-03-27
注記・抄録
博士論文
目次
- Contents / (0004.jp2)
- Abstruct / p1 (0007.jp2)
- Chapter 1.Cloning of PEX12 and PEX10 cDNA / p3 (0009.jp2)
- 1.1.Introduction / p4 (0010.jp2)
- 1.2.Results / p6 (0012.jp2)
- 1.3.Discussion / p12 (0018.jp2)
- Chapter 2.Cloning of Chinese hamster PEX12 and domain mapping of Pex 12p / p14 (0020.jp2)
- 2.1.Introduction / p15 (0021.jp2)
- 2.2.Results / p16 (0022.jp2)
- 2.3.Discussion / p21 (0027.jp2)
- Chapter 3.Interaction of RING peroxins / p23 (0029.jp2)
- 3.1.Introduction / p24 (0030.jp2)
- 3.2.Results / p25 (0031.jp2)
- 2.3.Discussion / p28 (0034.jp2)
- Materials and methods / p30 (0036.jp2)
- Cell lines and culture condition / p30 (0036.jp2)
- Antibodies / p30 (0036.jp2)
- DNA transfection / p30 (0036.jp2)
- Morphological analysis / p31 (0037.jp2)
- Construction of rat liver cDNA library / p31 (0037.jp2)
- Screening of complementing cDNA for ZP109 / p31 (0037.jp2)
- Cloning of human PEX12 cDNA / p32 (0038.jp2)
- Mutation analysis of PEX12 from CG-III patients fibroblasts / p32 (0038.jp2)
- Expression of epitope-tagged Pexl2p / p32 (0038.jp2)
- Cloning of human PEX10 cDNA / p33 (0039.jp2)
- Mutation analysis of PEX10 from CG-B fibroblasts / p33 (0039.jp2)
- Expression of epitope-tagged PexlOp / p34 (0040.jp2)
- Cloning and transfection of Chinese hamster PEX12 cDNA / p34 (0040.jp2)
- Mutation analysis of PEX12 in ZP104 and ZP109 / p35 (0041.jp2)
- N-terminal deletion mutants of rat Pexl2p / p35 (0041.jp2)
- Site-mutagenesis in RING finger of rat Pex12p / p36 (0042.jp2)
- C-terminal deletion mutants of rat Pex12p / p36 (0042.jp2)
- Yeast two-hybrid assay / p37 (0043.jp2)
- Construction for expression of fusion proteins / p39 (0045.jp2)
- Purification of recombinant proteins / p39 (0045.jp2)
- In vitro binding assay / p40 (0046.jp2)
- Immunoprecipitation of Flag-Pex10p / p41 (0047.jp2)
- Acknowledgments / p42 (0048.jp2)
- References / p43 (0049.jp2)
- Figure legends / p51 (0057.jp2)
- Tables / p58 (0064.jp2)
- Figures / p58 (0069.jp2)