DNA-binding peptides searched from the solid-phase combinatrial library with the use of the magnetic beads attaching the target duplex DNA 標的2本鎖DNA結合マグネティックビーズを用いた固相コンビナトリアルライブラリーからのDNA結合ペプチドの探索

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著者

    • Md. Rowshon Alam モハメッド ロウション アラム

書誌事項

タイトル

DNA-binding peptides searched from the solid-phase combinatrial library with the use of the magnetic beads attaching the target duplex DNA

タイトル別名

標的2本鎖DNA結合マグネティックビーズを用いた固相コンビナトリアルライブラリーからのDNA結合ペプチドの探索

著者名

Md. Rowshon Alam

著者別名

モハメッド ロウション アラム

学位授与大学

九州大学

取得学位

博士 (薬学)

学位授与番号

甲第5071号

学位授与年月日

2000-03-27

注記・抄録

博士論文

目次

  1. Contents / (0003.jp2)
  2. Abstract / p1 (0005.jp2)
  3. Chapter1 Introduction / p2 (0006.jp2)
  4. 1.1 General Aspects / p2 (0006.jp2)
  5. 1.2 Purpose of Study / p4 (0007.jp2)
  6. 1.3 Pentapeptide Library as a Source of DNA-Binding Ligands / p6 (0008.jp2)
  7. 1.4 Combinatorial Method / p7 (0008.jp2)
  8. 1.5 Pool and Split Strategy / p8 (0009.jp2)
  9. 1.6 Random Screening / p9 (0009.jp2)
  10. 1.7 Structures of Library and Magnetic Beads Used in the Random Screening / p10 (0010.jp2)
  11. 1.8 Binding Modes of Small Molecules to Duplex DNA / p11 (0010.jp2)
  12. Chapter2 Experimental Design and Synthesis / p14 (0012.jp2)
  13. 2.1 Design of Synthetic Linker Derivatized Duplex DNA / p14 (0012.jp2)
  14. 2.2 Synthesis of Oligonucleotide by Phosphoramidite Method / p17 (0013.jp2)
  15. 2.3 Synthesis of Linker-Derivatized Designed Duplex(3) / p19 (0014.jp2)
  16. 2.4 Preparation of Magnetic Beads Attaching the Target Duplex DNA(3) / p21 (0015.jp2)
  17. 2.5 Synthesis of a Combinatorial Library:"One-Sequence-Per-Bead" / p23 (0016.jp2)
  18. 2.6 Synthesis of Pentapeptide Library Beads / p26 (0018.jp2)
  19. Chapter3 Random Screening / p29 (0019.jp2)
  20. 3.1 Screening of the Pentapeptide Library Beads with the Target DNA-Conjugated Magnet Beads / p28 (0019.jp2)
  21. 3.2 Microphotograph of Complexes Between Library and Magnetic Beads / p29 (0019.jp2)
  22. Chapter4 DNA-Binding Pentapeptides / p31 (0020.jp2)
  23. 4.1 Determination of Sequences of the Selected Pentapeptide Beads / p31 (0020.jp2)
  24. 4.2 Analysis of Amino Acids of the Determined Peptide Sequences / p32 (0021.jp2)
  25. 4.3 Synthesis of Pentapeptides on the HMP Resin / p35 (0022.jp2)
  26. 4.4 Determination of Binding Parameters of Synthetic Pentapeptides and Ethidium Bromide(ETBr) / p37 (0023.jp2)
  27. 4.5 Effect of a Single Amino Residue of Pentapeptides on DNA Binding / p44 (0027.jp2)
  28. Chapter5 / p46 (0028.jp2)
  29. General Discussion / p46 (0028.jp2)
  30. Therapeutic Potential of Peptides / p50 (0030.jp2)
  31. Conclusion / p51 (0030.jp2)
  32. Acknowledgements / p52 (0031.jp2)
  33. Chapter6 Experimental / p54 (0032.jp2)
  34. 6.1 General / p54 (0032.jp2)
  35. 6.2 Synthesis of 2-Cyanoethyl N,N-diisopropylchlorophosphoramidite / p56 (0033.jp2)
  36. 6.3 Synthesis of(4,4'-Dimethoxytrityl)hexa(ethylene)gIycol 2-Cyanoethyl N,N-Diisipropylphosphoramidite / p57 (0033.jp2)
  37. 6.4 Synthesis of Amino Link 2 / p58 (0034.jp2)
  38. 6.5 Synthesis of Amino Link Conjugating Target Duplex DNA / p59 (0034.jp2)
  39. 6.6 Synthesis of Magnetic Beads Attaching the Target Duplex DNA / p61 (0035.jp2)
  40. 6.7 Synthesis of the Pentapeptide Library Beads by the "Pool & Split" Method / p64 (0037.jp2)
  41. 6.8 Selection Procedure of the Library Beads with the Target Conjugated Magnetic Beads / p66 (0038.jp2)
  42. 6.9 Synthesis of Pentapeptides on HMP Resin / p66 (0038.jp2)
  43. 6.10 Binding Experiments / p72 (0041.jp2)
  44. Chapter7 / p75 (0042.jp2)
  45. References / p75 (0042.jp2)
  46. Abbreviations / p81 (0045.jp2)
  47. List of Publications / p82 (0046.jp2)
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各種コード

  • NII論文ID(NAID)
    500000187593
  • NII著者ID(NRID)
    • 8000000187876
  • DOI(NDL)
  • NDL書誌ID
    • 000000351907
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション
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