Oxyhemoglobin-induced apoptosis in cultured endothelial cells 培養内皮細胞におけるオキシヘモグロビンによるアポトーシスの誘導

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著者

    • 荻原, 浩太郎 オギハラ, コウタロウ

書誌事項

タイトル

Oxyhemoglobin-induced apoptosis in cultured endothelial cells

タイトル別名

培養内皮細胞におけるオキシヘモグロビンによるアポトーシスの誘導

著者名

荻原, 浩太郎

著者別名

オギハラ, コウタロウ

学位授与大学

岡山大学

取得学位

博士 (医学)

学位授与番号

乙第3441号

学位授与年月日

2000-03-25

注記・抄録

博士論文

OBJECT: Oxyhemoglobin (OxyHb) is one of the most important spasmogens for cerebral vasospasm that follows aneurysmal subarachnoid hemorrhage. The cytotoxic effect of OxyHb has been documented in endothelial and smooth-muscle cells; however, the pattern of cell death--necrosis or apoptosis--as the final stage of cell damage has not been demonstrated. This study was undertaken to determine if OxyHb induces apoptotic changes in cultured bovine aortic endothelial cells. METHODS: Confluent bovine aortic endothelial cells were treated with OxyHb in a concentration- and time-dependent manner. Cell density was assayed by counting the number of cells that attached to culture dishes after exposure to OxyHb. To identify apoptotic changes, the investigators used three specific methods: DNA fragmentation (electrophoreses), the apoptotic body (transmission electron microscopy), and cleavage of poly (adenosine diphosphate ribose) polymerase (PARP [Western blotting]). CONCLUSIONS: Oxyhemoglobin decreased cell density in a concentration- and time-dependent manner. Analysis of DNA showed a pattern of internucleosomal cleavage characteristic of apoptosis (DNA ladder). Transmission electron microscopy demonstrated condensation of nuclei and apoptotic bodies in OxyHb-treated endothelial cells. Western blotting with the PARP antibody revealed that the 116-kD PARP was cleaved to the 85-kD apoptosis-related fragment. These results for the first time demonstrated that the OxyHb induces apoptosis in cultured endothelial cells.

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各種コード

  • NII論文ID(NAID)
    500000189376
  • NII著者ID(NRID)
    • 8000000189659
  • DOI(NDL)
  • 本文言語コード
    • jpn
  • NDL書誌ID
    • 000000353690
  • データ提供元
    • 機関リポジトリ
    • NDL ONLINE
    • NDLデジタルコレクション
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