Analysis of binding interaction between chaperonin GroEL and substrate protein by fluorescence correlation spectroscopy 蛍光相関分光法によるシャペロニンGroEL-基質蛋白質間相互作用の解析

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Author

    • 白, 燦基 ベック, チャンギ

Bibliographic Information

Title

Analysis of binding interaction between chaperonin GroEL and substrate protein by fluorescence correlation spectroscopy

Other Title

蛍光相関分光法によるシャペロニンGroEL-基質蛋白質間相互作用の解析

Author

白, 燦基

Author(Another name)

ベック, チャンギ

University

北海道大学

Types of degree

博士 (理学)

Grant ID

甲第5002号

Degree year

2000-03-24

Note and Description

博士論文

Table of Contents

  1. Contents / p1 (0003.jp2)
  2. Abbreviations / p2 (0004.jp2)
  3. 1.Introduction / p3 (0005.jp2)
  4. 1.1 Introduction / p3 (0005.jp2)
  5. 1.2 Purpose of this study / p9 (0011.jp2)
  6. 2.Fluorescence correlation spectroscopy(FCS) / p14 (0016.jp2)
  7. 2.1 Introduction / p14 (0016.jp2)
  8. 2.2 Conceptual basis and fundamental setup of FCS / p15 (0017.jp2)
  9. 2.3 Theoretical principle of FCS / p17 (0019.jp2)
  10. 2.4 Fluorescence autocorrelation function(FAF) for translational diffusion / p22 (0024.jp2)
  11. 2.5 Analysis of molecular interaction by FCS / p25 (0027.jp2)
  12. 3.Measurement of FAFs of proteins / p33 (0035.jp2)
  13. 3.1 Sample preparation / p33 (0035.jp2)
  14. 3.2 Experiments / p37 (0039.jp2)
  15. 3.3 Results / p39 (0041.jp2)
  16. 3.4 Discussion / p44 (0046.jp2)
  17. 4.Binding interaction of chaperonin,GroEL,with substrate protein / p60 (0062.jp2)
  18. 4.1 Experiments / p60 (0062.jp2)
  19. 4.2 Results / p62 (0064.jp2)
  20. 4.3 Discussion / p66 (0068.jp2)
  21. 5.Effect of electrostatic interaction on the binding of GroEL to substrate / p81 (0083.jp2)
  22. 5.1 Experiments / p81 (0083.jp2)
  23. 5.2 Results / p82 (0084.jp2)
  24. 5.3 Discussion / p86 (0088.jp2)
  25. 6.Conclusion and prospects / p100 (0102.jp2)
  26. References / p104 (0106.jp2)
  27. Acknowledgments / (0114.jp2)
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Codes

  • NII Article ID (NAID)
    500000189445
  • NII Author ID (NRID)
    • 8000000189728
  • DOI(NDL)
  • NDLBibID
    • 000000353759
  • Source
    • NDL ONLINE
    • NDL Digital Collections
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