A study of cytochrome c fragment complex and four species of lysozyme variants lacking a specific disulfide bridge

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著者

    • 横田, 篤 ヨコタ, アツシ

書誌事項

タイトル

A study of cytochrome c fragment complex and four species of lysozyme variants lacking a specific disulfide bridge

著者名

横田, 篤

著者別名

ヨコタ, アツシ

学位授与大学

関西学院大学

取得学位

博士 (理学)

学位授与番号

甲第117号

学位授与年月日

2000-03-17

注記・抄録

博士論文

目次

  1. Abstract / (0003.jp2)
  2. Contents / (0006.jp2)
  3. 1 Introduction / p1 (0010.jp2)
  4. 1-1 Protein Folding Problem / p1 (0010.jp2)
  5. 1-2 Folding of Lysozyme / p6 (0015.jp2)
  6. 1-3 Significance of Site Mutation / p10 (0019.jp2)
  7. 1-4 Folding of Cytochrome c(cyt c) / p12 (0021.jp2)
  8. 2 Methods and Sample Preparations / p18 (0027.jp2)
  9. 2-1 Isothermal Titration Calorimetry(ITC) / p18 (0027.jp2)
  10. 2-2 Differential Scanning Calorimetry(DSC) / p24 (0033.jp2)
  11. 2-3 Preparation of 3SS-variants of lysozyme / p29 (0038.jp2)
  12. 2-4 Limited Digestion of Tuna Cytochrome c by V8 Protease:Preparation of tuna cyt c fragments / p34 (0043.jp2)
  13. 3 Structure and Stability of Four Species of Three-disulfide Variant of Hen Lysozyme / p37 (0046.jp2)
  14. 3-1 Introduction / p37 (0046.jp2)
  15. 3-2 Four Species of Lysozyme Variant Lacking a Specific Disulfide Bridge / p38 (0047.jp2)
  16. 3-3 CD Spectra of 3SS-Variants of Lysozyme / p41 (0050.jp2)
  17. 3-4 NMR Spectra of 3SS-Variants of Lysozyme / p42 (0051.jp2)
  18. 3-5 DSC measurement of 3SS-Variants of Lysozyme / p44 (0053.jp2)
  19. 3-6 Calorimetric Titration of triNAG into 3SS-Variants of Lysozyme / p47 (0056.jp2)
  20. 3-7 Conclusion / p51 (0060.jp2)
  21. 3-8 Materials and Methods / p52 (0061.jp2)
  22. 4 Transition State in the Folding-Unfolding Reaction of Four Species of 3SS-Variants of Lysozyme / p54 (0063.jp2)
  23. 4-1 Introduction / p54 (0063.jp2)
  24. 4-2 Unfolding Transition Curves of 3SS-Variants of Lysozyme / p57 (0066.jp2)
  25. 4-3 Folding Kinetics of wtLYZ,metLYYZ and rcmLYZ / p61 (0070.jp2)
  26. 4-4 Folding Kinetics of 3SS-Variants of Lysozyme / p65 (0074.jp2)
  27. 4-5 Unfolding Kinetics of 3SS-Variants of Lysozyme / p68 (0077.jp2)
  28. 4-6 Discussion / p72 (0081.jp2)
  29. 4-7 Conclusion:The Role of Each Disulfide Bridge / p77 (0086.jp2)
  30. 4-8 Materials and Methods / p79 (0088.jp2)
  31. 5 Thermodynamics of the Reconstitution of Tuna Cytochrome c from Two Peptide Fragments / p82 (0091.jp2)
  32. 5-1 Introduction / p82 (0091.jp2)
  33. 5-2 Fragment-Complex Formation between N-and C-Fragments / p84 (0093.jp2)
  34. 5-3 Proton NMR Spectra of the Fragment-Complex / p89 (0098.jp2)
  35. 5-4 Calorimetric Titration of N-Fragment into C-Fragment / p92 (0101.jp2)
  36. 5-5 Dependence of ln Kb and ΔHb on pH / p93 (0102.jp2)
  37. 5-6 Dependence of ln Kb and ΔHb on Temperature / p96 (0105.jp2)
  38. 5-7 Thermodynamics of Tuna Cyt c Unfolding:DSC Measurements / p100 (0109.jp2)
  39. 5-8 Discussion / p103 (0112.jp2)
  40. 5-9 Appendix:Dependence of observed binding constants on temperature / p111 (0120.jp2)
  41. 5-10 Materials and Methods / p113 (0122.jp2)
  42. 6 References / p116 (0125.jp2)
  43. 7 Acknowledgement / p123 (0132.jp2)
  44. Thermodynamics of the reconstitution of tuna cytochrome c from two peptide fragments / p1717 (0134.jp2)
  45. Folding-unfolding processes of four species of 3SS-vanant of lysozyme-the role of an individual disulfide bridge / p163 (0148.jp2)
  46. The Transition State in the Folding-Unfolding Reaction of Four Species of Three-disulfide Variant of Hen Lysozyme:The Role of Each Disulfide Bridge / p1 (0161.jp2)
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各種コード

  • NII論文ID(NAID)
    500000189756
  • NII著者ID(NRID)
    • 8000000190039
  • DOI(NDL)
  • NDL書誌ID
    • 000000354070
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション
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