A study of cytochrome c fragment complex and four species of lysozyme variants lacking a specific disulfide bridge
この論文にアクセスする
この論文をさがす
著者
書誌事項
- タイトル
-
A study of cytochrome c fragment complex and four species of lysozyme variants lacking a specific disulfide bridge
- 著者名
-
横田, 篤
- 著者別名
-
ヨコタ, アツシ
- 学位授与大学
-
関西学院大学
- 取得学位
-
博士 (理学)
- 学位授与番号
-
甲第117号
- 学位授与年月日
-
2000-03-17
注記・抄録
博士論文
目次
- Abstract / (0003.jp2)
- Contents / (0006.jp2)
- 1 Introduction / p1 (0010.jp2)
- 1-1 Protein Folding Problem / p1 (0010.jp2)
- 1-2 Folding of Lysozyme / p6 (0015.jp2)
- 1-3 Significance of Site Mutation / p10 (0019.jp2)
- 1-4 Folding of Cytochrome c(cyt c) / p12 (0021.jp2)
- 2 Methods and Sample Preparations / p18 (0027.jp2)
- 2-1 Isothermal Titration Calorimetry(ITC) / p18 (0027.jp2)
- 2-2 Differential Scanning Calorimetry(DSC) / p24 (0033.jp2)
- 2-3 Preparation of 3SS-variants of lysozyme / p29 (0038.jp2)
- 2-4 Limited Digestion of Tuna Cytochrome c by V8 Protease:Preparation of tuna cyt c fragments / p34 (0043.jp2)
- 3 Structure and Stability of Four Species of Three-disulfide Variant of Hen Lysozyme / p37 (0046.jp2)
- 3-1 Introduction / p37 (0046.jp2)
- 3-2 Four Species of Lysozyme Variant Lacking a Specific Disulfide Bridge / p38 (0047.jp2)
- 3-3 CD Spectra of 3SS-Variants of Lysozyme / p41 (0050.jp2)
- 3-4 NMR Spectra of 3SS-Variants of Lysozyme / p42 (0051.jp2)
- 3-5 DSC measurement of 3SS-Variants of Lysozyme / p44 (0053.jp2)
- 3-6 Calorimetric Titration of triNAG into 3SS-Variants of Lysozyme / p47 (0056.jp2)
- 3-7 Conclusion / p51 (0060.jp2)
- 3-8 Materials and Methods / p52 (0061.jp2)
- 4 Transition State in the Folding-Unfolding Reaction of Four Species of 3SS-Variants of Lysozyme / p54 (0063.jp2)
- 4-1 Introduction / p54 (0063.jp2)
- 4-2 Unfolding Transition Curves of 3SS-Variants of Lysozyme / p57 (0066.jp2)
- 4-3 Folding Kinetics of wtLYZ,metLYYZ and rcmLYZ / p61 (0070.jp2)
- 4-4 Folding Kinetics of 3SS-Variants of Lysozyme / p65 (0074.jp2)
- 4-5 Unfolding Kinetics of 3SS-Variants of Lysozyme / p68 (0077.jp2)
- 4-6 Discussion / p72 (0081.jp2)
- 4-7 Conclusion:The Role of Each Disulfide Bridge / p77 (0086.jp2)
- 4-8 Materials and Methods / p79 (0088.jp2)
- 5 Thermodynamics of the Reconstitution of Tuna Cytochrome c from Two Peptide Fragments / p82 (0091.jp2)
- 5-1 Introduction / p82 (0091.jp2)
- 5-2 Fragment-Complex Formation between N-and C-Fragments / p84 (0093.jp2)
- 5-3 Proton NMR Spectra of the Fragment-Complex / p89 (0098.jp2)
- 5-4 Calorimetric Titration of N-Fragment into C-Fragment / p92 (0101.jp2)
- 5-5 Dependence of ln Kb and ΔHb on pH / p93 (0102.jp2)
- 5-6 Dependence of ln Kb and ΔHb on Temperature / p96 (0105.jp2)
- 5-7 Thermodynamics of Tuna Cyt c Unfolding:DSC Measurements / p100 (0109.jp2)
- 5-8 Discussion / p103 (0112.jp2)
- 5-9 Appendix:Dependence of observed binding constants on temperature / p111 (0120.jp2)
- 5-10 Materials and Methods / p113 (0122.jp2)
- 6 References / p116 (0125.jp2)
- 7 Acknowledgement / p123 (0132.jp2)
- Thermodynamics of the reconstitution of tuna cytochrome c from two peptide fragments / p1717 (0134.jp2)
- Folding-unfolding processes of four species of 3SS-vanant of lysozyme-the role of an individual disulfide bridge / p163 (0148.jp2)
- The Transition State in the Folding-Unfolding Reaction of Four Species of Three-disulfide Variant of Hen Lysozyme:The Role of Each Disulfide Bridge / p1 (0161.jp2)