モルモットマクロファージを認識する新しいCD68モノクローナル抗体の作製と解析 Production and characterization of a new CD68 monoclonal antibody recognizing guinea pig macrophage

博士論文

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学位論文(Thesis)

Macrophages are present in nearly all tissues and organs of the body. They can be identified immunohistochemically by virtue of the presence of monocyte/macrophage-associated antigens such as CD11b, CD14, CD68, CD163, CD169, and CD204. Among these molecules, CD68 is most widely distributed in monocyte/macrophages at various differentiation stages and activation states. Antibodies recognizing CD68 have been used as some of the best reagents for labeling macrophages in tissues. Many antibodies recognize human CD68 antigens, however, only a few antibodies reportedly recognize CD68 in animals, especially guinea pigs.

本研究では、免疫沈降法を用いてPM-1Kの抗原解析を行うとともに、モルモットにおける反応性を系統的に解析し、PM-1Kのモルモットへの応用可能性について、検証を加えた。