発光キノコ、ヤコウタケ生物発光機構研究 Study on Bioluminescence mechanism of luminous fungus Mycena chlorophos

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著者

    • 森, 憲一 モリ, ケンイチ

書誌事項

タイトル

発光キノコ、ヤコウタケ生物発光機構研究

タイトル別名

Study on Bioluminescence mechanism of luminous fungus Mycena chlorophos

著者名

森, 憲一

著者別名

モリ, ケンイチ

学位授与大学

電気通信大学

取得学位

博士 (理学)

学位授与番号

甲第624号

学位授与年月日

2011-03-24

注記・抄録

博士論文

2010

Bioluminescent fungi are widely distributed on land and most belong to the class ofBasidomycetes. Light of about 530 nm wavelength maximum is emitted continuously.Despite many efforts to determine the mechanism of light emission in fungi, the detailedmolecular basis for the light-emitting process remains unclear.Mycena chlorophos (yako-take in Japanese) is distributed in subtropical areas inJapan including on Hachijo Island and the Bonin (Ogasawara in Japanese) Islands. Innature, the fruiting bodies of M. chlorophos develop mainly on decaying petioles of apalm Phenix roeberenii, and only on the day after rain falls during the rainy season(June/July and September/October). The limited distribution and the limited season forthe formation of the fruiting bodies have made the chemical study of thebioluminescence mechanism difficult.Cultivation of the fruiting bodies using peat moss as a solid medium was studied andwe found that the peat moss medium was satisfactory for the production of fruitingbodies of M. chlorophos.The characteristics of the bioluminescence by using cultivated fruiting bodies of M.chlorophos was investigated under various conditions to obtain valuable information forthe isolation of the luciferin and luciferase.Long-lasting light emission was observed only in fresh fruiting bodies, not in thefrozen and freeze-dried samples.Although clear correlation between the light emission intensity of the fruiting bodiesand the buffer pH was not observed, the pH change of acetate and citrate buffers gavethe reverse effect against the light emission intensity. A marked change in light intensitywas observed in acetate buffer: the light intensity in acetate buffer at pH 4 decreasedrapidly within 10 min, while the initial light intensity of the pileus was maintained for along time at pH 6. Acetic acid inhibited M. chlorophos bioluminescence, whereassodium acetate did not. The light emission of the fresh fruiting bodies could be stoppedwith pH 4 acetate buffer, and could be recovered at pH 6. Thus, the luciferin might beisolated from the fruiting bodies whose the light-emission was inhibited.ヤコウタケは日本の八丈島など亜熱帯気候の地域に分布している発光キノコである。子実体からの発光量が多く、1~3 日間持続して緑色に発光する。ヤコウタケなどの発光菌類は発光機構について未解明な部分が多い。今回、発光機構解明研究の一環として、ヤコウタケ子実体の大量確保のための実験室栽培を試みた。その結果ヤコウタケ子実体の簡易栽培法を確立し、研究材料の大量確保に目処を付けることができた。栽培した子実体の発光特性を検討した所、子実体の発光を可逆的に制御することができることを見出し、ヤコウタケ生物発光に関係する物質(ルシフェリン)の単離に道を拓くことが出来た。

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各種コード

  • NII論文ID(NAID)
    500000547452
  • NII著者ID(NRID)
    • 8000000549544
  • 本文言語コード
    • jpn
  • NDL書誌ID
    • 023263048
  • データ提供元
    • 機関リポジトリ
    • NDL ONLINE
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