Overexpression of the adiponectin gene mimics the metabolic and stress resistance effects of calorie restriction, but not the anti-tumor effect アディポネクチン遺伝子の過剰発現はカロリー制限による代謝適応反応およびストレス耐性作用を模倣するが抗腫瘍効果は示さない

Adiponectin (Adipoq), a peptide hormone secreted from the white adipose tissue, may play a role in the antiaging and/or anti-tumor effects of calorie restriction (CR). We analyzed metabolic traits in Adipoq geneoverexpressing mice fed ad libitumwith a regular diet (RD) or a high-fat diet (HFD), or fed 30% CR of RD initiated at 12weeks of age. Adipoq-RD and -HFD mice at 6 months of age showed reduced blood glucose and insulin concentrations, and thus increased insulin sensitivity, compared withWTmice fed a RD or a HFD. In the epididymal white adipose tissue in Adipoq mice, senescence-like changes such as upregulation of p53 protein and of biomarkers of inflammation, Cd68 and Ccl2 mRNA, were ameliorated compared withWT-RD and WT-HFD mouse tissues. Resistance to stress induced by lipopolysaccharide was also strengthened in Adipoq mice compared with WT mice. These metabolic changes and stress resistance were also noted in the WT-CR mice, suggestingthat Adipoq plays a part in the effect of CR. In contrast, in an allograft tumor growth model, tumor growth was not inhibited in Adipoq mice. The present findings suggest that Adipoq plays a part in the anti-aging, but not in the anti-tumor, effects of CR.

長崎大学学位論文 学位記番号:博(医歯薬)甲第786号 学位授与年月日:平成27年8月5日

Author: Ryotaro Kamohara, Haruyoshi Yamaza, Tomoshi Tsuchiya, Toshimitsu Komatsu, Seongjoon Park, Hiroko Hayashi, Takuya Chiba, Ryoichi Mori, Shuichi Otabe, Kentaro Yamada, Takeshi Nagayasu, Isao Shimokawa

Citation: Experimental Gerontology, 64, pp.46-54; 2015

Nagasaki University (長崎大学), 博士(医学) (2015-08-05)

Adiponectin (Adipoq), a peptide hormone secreted from the white adipose tissue, may play a role in the antiaging and/or anti-tumor effects of calorie restriction (CR). We analyzed metabolic traits in Adipoq geneoverexpressing mice fed ad libitumwith a regular diet (RD) or a high-fat diet (HFD), or fed 30% CR of RD initiated at 12weeks of age. Adipoq-RD and -HFD mice at 6 months of age showed reduced blood glucose and insulin concentrations, and thus increased insulin sensitivity, compared withWTmice fed a RD or a HFD. In the epididymal white adipose tissue in Adipoq mice, senescence-like changes such as upregulation of p53 protein and of biomarkers of inflammation, Cd68 and Ccl2 mRNA, were ameliorated compared withWT-RD and WT-HFD mouse tissues. Resistance to stress induced by lipopolysaccharide was also strengthened in Adipoq mice compared with WT mice. These metabolic changes and stress resistance were also noted in the WT-CR mice, suggesting that Adipoq plays a part in the effect of CR. In contrast, in an allograft tumor growth model, tumor growth was not inhibited in Adipoq mice. The present findings suggest that Adipoq plays a part in the anti-aging, but not in the anti-tumor, effects of CR.

長崎大学学位論文 [学位記番号]博（医歯薬）甲第786号 [学位授与年月日]平成27年8月5日

(2015-08-05)