非がん細胞とがん細胞の鑑別における紫外・可視顕微分光法の有用性

type:Thesis

In this study, my purpose is to clarify whether Ultraviolet- and Visible- Light Microscopic Spectroscopy method (UV-Vis MS) is a useful technique for discrimination of non-cancer cells and cancer cells. This manuscript is composed of three chapters.In the first chapter, we examined the usefulness of UV-MS on unstained cells by liquid-based cytology (LBC). Cultured cells were used as the samples: 100 non-cancer cells and 200 cancer cells. On UV-MS, transmittance data at 260, 280, 300, 320, and 340 nm were extracted from the transmittance spectrum of 260-350 nm UV wavelengths and analyzed. As a result, The transmittance was lower in cancer than in non-cancer cells at all wavelengths (p <0.01).Thus, it was revealed that substance in cancer cells that absorbs ultraviolet light is rich. The result of discriminant analysis showed that determine predictive value was 96.3% (Z = 0.61 × 300 nm transmittance -44.02).In the second chapter, we examined the usefulness of Vis-MS on Papanicolaou stained cells by liquid-based cytology (LBC). The cells of the same type in the first chapter were smeared on a glass slide. On Vis-MS, transmittance data at 530, 580, 630 nm from the transmittance spectrum of400-650-nm Vis, and 530 nm/580 nm and 630 nm/560 nm transmittance ratio were extracted and analyzed. As a result, the transmittance at 580 nm was lower in cancer than in non-cancer cells (p <0.01). 530 nm/580 nm transmittance ratio was high in cancer than in non -cancer cells (p <0.01), and 630 nm/560 nm transmittance ratio was lower in cancer than in non-cancer cells (p <0.01). Binominal logistic regression analysis showed that the variables do not have a multicollinearity. Using the forward chapter method, 580 nm transmission values and 630 nm / 580 nm transmittance ratio were selected. Binomial logistic regression equation was obtained and the determine predictive value was 96.3%.The last chapter, I examined the usefulness of UV-Vis MS on tissue section. Samples were well- (G1) and poorly (G3) differentiated types of endometrial endometrioid adenocarcinoma. We analyzed the wavelength of 320 nm, 260 nm, 280 nm, and 300 nm on unstained samples by UV-MS. In the same area of the same section, we analyzed the wavelength of, 560 nm, 520 nm, and 540 nm on the HE stained samples by the Vis-MS. We investigated that cononical correlation analysis was useful for determination of the G1 and G3. As a result, it was suggested that the nuclear color changes in G3 was caused by ultraviolet absorbing material unspecified.In conculsion, it was clarified that UV-Vis MS is an objective and effective tool for discrimination of cancer cells. We will apply and develop it as an objective index for the diagnosis and prognosis of patients with various cancers.

In this study, my purpose is to clarify whether Ultraviolet- and Visible- Light Microscopic Spectroscopy method (UV-Vis MS) is a useful technique for discrimination of non-cancer cells and cancer cells. This manuscript is composed of three chapters. In the first chapter, we examined the usefulness of UV-MS on unstained cells by liquid-based cytology (LBC). Cultured cells were used as the samples: 100 non-cancer cells and 200 cancer cells. On UV-MS, transmittance data at 260, 280, 300, 320, and 340 nm were extracted from the transmittance spectrum of 260-350 nm UV wavelengths and analyzed. As a result, The transmittance was lower in cancer than in non-cancer cells at all wavelengths (p <0.01).Thus, it was revealed that substance in cancer cells that absorbs ultraviolet light is rich. The result of discriminant analysis showed that determine predictive value was 96.3% (Z = 0.61 × 300 nm transmittance -44.02). In the second chapter, we examined the usefulness of Vis-MS on Papanicolaou stained cells by liquid-based cytology (LBC). The cells of the same type in the first chapter were smeared on a glass slide. On Vis-MS, transmittance data at 530, 580, 630 nm from the transmittance spectrum of 400-650-nm Vis, and 530 nm/580 nm and 630 nm/560 nm transmittance ratio were extracted and analyzed. As a result, the transmittance at 580 nm was lower in cancer than in non-cancer cells (p <0.01). 530 nm/580 nm transmittance ratio was high in cancer than in non -cancer cells (p <0.01), and 630 nm/560 nm transmittance ratio was lower in cancer than in non-cancer cells (p <0.01). Binominal logistic regression analysis showed that the variables do not have a multicollinearity. Using the forward chapter method, 580 nm transmission values and 630 nm / 580 nm transmittance ratio were selected. Binomial logistic regression equation was obtained and the determine predictive value was 96.3%. The last chapter, I examined the usefulness of UV-Vis MS on tissue section. Samples were well- (G1) and poorly (G3) differentiated types of endometrial endometrioid adenocarcinoma. We analyzed the wavelength of 320 nm, 260 nm, 280 nm, and 300 nm on unstained samples by UV-MS. In the same area of the same section, we analyzed the wavelength of, 560 nm, 520 nm, and 540 nm on the HE stained samples by the Vis-MS. We investigated that cononical correlation analysis was useful for determination of the G1 and G3. As a result, it was suggested that the nuclear color changes in G3 was caused by ultraviolet absorbing material unspecified. In conculsion, it was clarified that UV-Vis MS is an objective and effective tool for discrimination of cancer cells. We will apply and develop it as an objective index for the diagnosis and prognosis of patients with various cancers.