Beneficial effect of D-allose for isolated islet culture prior to islet transplantation

著者

    • 柏木, 裕貴

書誌事項

タイトル

Beneficial effect of D-allose for isolated islet culture prior to islet transplantation

著者名

柏木, 裕貴

学位授与大学

香川大学

取得学位

博士(医学)

学位授与番号

甲第666号

学位授与年月日

2017-03-24

注記・抄録

BackgroundPretransplant restoration of islets damaged during isolation remains to be solved. In this study, we examined the effect of D‐allose on islets isolated from rat pancreata prior to islet transplantation.

MethodsRat islets isolated from fresh pancreata were cultured overnight in Roswell Park Memorial Institute 1640 solution in the absence (group 1) or presence (group 2) of D‐allose. Then we assessed stimulation index of insulin, and cure rate after islet transplantation to diabetic nude mice. We also measured malondialdehyde level and caspase 3 activity of islets after the overnight culture for assessment of the oxidative stress and the apoptosis.

ResultsD‐allose significantly improved insulin secretion of islets. The stimulation index in group 2 was significantly higher than in group 1. Cure rate after transplantation in group 2 was higher than in group 1 especially in the first week. The malondialdehyde level in group 2 was significantly lower than in group 1. But the caspase 3 activities in both groups did not differ.

ConclusionsD‐allose treatment of isolated islet culture prior to transplantation restored islet function and increased successful transplant rate. The results of this study suggested that D‐allose improved function of damaged islets through its anti‐oxidative activity.

Background Pretransplant restoration of islets damaged during isolation remains to be solved. In this study, we examined the effect of D‐allose on islets isolated from rat pancreata prior to islet transplantation.

Methods Rat islets isolated from fresh pancreata were cultured overnight in Roswell Park Memorial Institute 1640 solution in the absence (group 1) or presence (group 2) of D‐allose. Then we assessed stimulation index of insulin, and cure rate after islet transplantation to diabetic nude mice. We also measured malondialdehyde level and caspase 3 activity of islets after the overnight culture for assessment of the oxidative stress and the apoptosis.

Results D‐allose significantly improved insulin secretion of islets. The stimulation index in group 2 was significantly higher than in group 1. Cure rate after transplantation in group 2 was higher than in group 1 especially in the first week. The malondialdehyde level in group 2 was significantly lower than in group 1. But the caspase 3 activities in both groups did not differ.

Conclusions D‐allose treatment of isolated islet culture prior to transplantation restored islet function and increased successful transplant rate. The results of this study suggested that D‐allose improved function of damaged islets through its anti‐oxidative activity.

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各種コード

  • NII論文ID(NAID)
    500001039405
  • NII著者ID(NRID)
    • 8000001618224
  • DOI
  • 本文言語コード
    • eng
  • データ提供元
    • 機関リポジトリ
    • NDLデジタルコレクション
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