Antibacterial activity of phellodendron bark against Streptococcus mutans オウバクの Streptococcus mutans に対する抗菌効果
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著者
書誌事項
- タイトル
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Antibacterial activity of phellodendron bark against Streptococcus mutans
- タイトル別名
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オウバクの Streptococcus mutans に対する抗菌効果
- 著者名
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辻井, 利弥
- 学位授与大学
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鹿児島大学
- 取得学位
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博士(歯学)Doctor of Philosophy in Dental Science
- 学位授与番号
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甲総研第569号
- 学位授与年月日
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2020-08-20
注記・抄録
Streptococcus mutans is a major cause of tooth decay due to its promotion of biofilm formation and acid production. Several plant extracts have been reported to have multiple biological activities such as anti‐inflammation and antibacterial effects. This study investigated the antibacterial activity of three plant extracts, phellodendron bark (PB), yucca, and black ginger, and found that PB had a stronger effect than the other extracts. Then, the minimum inhibitory concentration (MIC) of PB against 100 S. mutans strains was investigated. The MIC range of PB was 9.8–312.5 µg/mL. PB suppressed the growth kinetics of S. mutans in a dose‐dependent manner, even at sub‐MICs of PB. Then, we investigated the effect of PB on S. mutans virulence. The PB suppressed biofilm formation at high concentrations, although PB did not affect the expression of glucosyltransferase genes. Additionally, PB suppressed the decrease in pH from adding an excess of glucose. The expression of genes responsible for acid production was increased by the addition of excess glucose without PB, whereas their expression levels were not increased in the presence of 1× and 2× MIC of PB. Although PB showed a bacteriostatic effect on planktonic S. mutans cells, it was found that more than 2× MIC of PB showed a partial bactericidal effect on biofilm cells. In conclusion, PB not only showed antibacterial activity against S. mutans but also decreased the cariogenic activity in S. mutans.
Toshiya Tsujii, Miki Kawada‐Matsuo, Hirono Migita, Kouji Ohta, Yuichi Oogai, Youichi Yamasaki, Hitoshi KomatsuzawaAntibacterial activity of phellodendron bark against Streptococcus mutansMicrobiology and Immunology 64(6) p.424-434 2020https://doi.org/10.1111/1348-0421.12787
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