Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice. アルサニル酸を用いたマウス内耳破壊後の前庭代償過程

収集根拠 : 博士論文（自動収集）

資料形態 : テキストデータ

コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文

When vestibular function is lost, vestibular compensation works for the reacquisition of body balance. For the study of vestibular dysfunction and vestibular compensation, surgical or chemical labyrinthectomy has been performed in various animal species. In the present study, we performed chemical labyrinthectomy using arsanilic acid in mice and investigated the time course of vestibular compensation through behavioral observations and histological studies. The surgical procedures required only paracentesis and storage of 50 µL of p-arsanilic acid sodium salt solution in the tympanic cavity for 5 min. From behavioral observations, vestibular functions were worst at 2 days and recovered by 7 days after surgery. Spontaneous nystagmus appeared at 1 day after surgery with arsanilic acid and disappeared by 2 days. Histological studies revealed specific damage to the vestibular endorgans. In the ipsilateral spinal vestibular nucleus, the medial vestibular nucleus, and the contralateral prepositus hypoglossal nucleus, a substantial number of c-Fos-immunoreactive cells appeared by 1 day after surgery with arsanilic acid, with a maximum increase in number by 2 days and complete disappearance by 7 days. Taken together, these findings indicate that chemical labyrinthectomy with arsanilic acid and the subsequent observation of vestibular compensation is a useful strategy for elucidation of the molecular mechanisms underlying vestibular pathophysiologies.

博士（医学）・甲第742号・令和2年3月16日

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

identifier:Brain sciences Vol.9 No.11 Article No.329 (2019 Nov)

identifier:20763425

identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3736

identifier:Brain sciences, 9(11): Article No.329

開始ページ : Article No.329