A Novel Metalloprotease from Gloydius halys Venom Induces Endothelial Cell Apoptosis through Its Protease and Disintegrin-Like Domains
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- You Weon-Kyoo
- Dept. of Biochemistry, College of Science, Yonsei University
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- Seo Hyun-Ji
- Dept. of Biochemistry, College of Science, Yonsei University
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- Chung Kwang-Hoe
- Cardiovascular Research Institute, Yonsei University
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- Kim Doo-Sik
- Dept. of Biochemistry, College of Science, Yonsei University
書誌事項
- タイトル別名
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- A Novel Metalloprotease from <i>Gloydius halys</i> Venom Induces Endothelial Cell Apoptosis through Its Protease and Disintegrin-Like Domains
- Novel Metalloprotease from Gloydius halys Venom Induces Endothelial Cell Apoptosis through Its Protease and Disintegrin Like Domains
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抄録
A novel hemorrhagic metalloprotease, halysase, isolated from the snake venom of Gloydius halys induces apoptosis in endothelial cells. The purified metalloprotease is a monomeric glycoprotein with an isoelectric point of 4. 8. Analysis of the cDNA sequence encoding halysase revealed that the enzyme consists of multifunctional domains including a proprotein domain, a protease domain, a disintegrin-like domain and a cysteine-rich domain. The metalloprotease has a DECD sequence in the disintegrin-like domain instead of the typical RGD sequence. Halysase strongly inhibits proliferation of human umbilical vein endothelial cells in a dose-dependent manner as well as adhesion of the cells to extracellular matrix proteins. The enzyme specifically hydrolyzes not only extracellular matrix proteins such as fibronectin, vitronectin, and type IV collagen, but also integrins α1β1 and α5α1. The apoptosis of endothelial cells induced by halysase is closely associated with activation of caspase-3 and decreased level of Bcl-XL/Bax. Apohalysase, which lacks metalloprotease activity, is also able to induce the apoptosis. Several lines of experimental evidence suggest that the protease domain and the disintegrin-like domain of halysase cooperatively contribute to the induction of endothelial cell apoptosis.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 134 (5), 739-749, 2003
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679943211776
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- NII論文ID
- 130003534622
- 50000366654
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD2cXpvVCqtg%3D%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 6781760
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- PubMed
- 14688240
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可