Development and Characterization of Conditionally Immortalized Gastric Epithelial Cell Lines from Transgenic Rats Harboring Temperature-Sensitve Simian Virus 40 Large T-antigen Gene

• Tabuchi Yoshiaki

  Molecular Genetics Research Center

• Arai Yuko

  Faculty of Pharmaceutical Science

• Ohta Shoichiro

  Faculty of Medicine, Toyama Medical and Pharmaceutical University

• Shioya Hiroki

  Faculty of Pharmaceutical Science

• Takahashi Ri-ichi

  YS New Technology Institute Inc.

• Ueda Masatsugu

  YS New Technology Institute Inc.

• Takeguchi Noriaki

  Faculty of Pharmaceutical Science

• Asano Shinji

  Molecular Genetics Research Center

• Obinata Masuo

  Institute of Development, Aging and Cancer, Tohoku University

書誌事項

タイトル別名

• Development and Characterization of Conditionally Immortalized Gastric Epithelial Cell Lines from Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene.

抄録

Conditionally immortalized gastric epithelial cell lines were established from transgenic rats harboring temperature-sensitive simian virus 40 (tsSV40) large T-antigen gene. Gastric mucosal cells and epithelial tissues isolated from the stomach of the transgenic rats were cultured at permissive temperature (33°C), and proliferative cells were cloned by colony formation. Six cell lines (designated as RGE1-01, RGE1-02, RGE1-03, RGE1-21, RGE1-22 and RGE2-01) showing epithelial-like morphology have been established. All cells grew at 33°C, but did not at nonpermissive temperature (39°C). High expression level of large T-antigen in the nuclei was observed at 33°C, whereas the expression level was gradually decreased in a time-dependent manner at 39°C. These results suggest that the temperature-sensitive growth characteristics arise as a result of a function of the tsSV40 large T-antigen. None of the cell lines were transformed as judged by anchorage-independent growth assay. Immunocytochemical findings indicated that all cells expressed epithelial cell markers including cytoskeletal (cytokeratin and actin), basement membrane (laminin and collagen type IV) and junctional complex (ZO-1 and desmoplakin I+II) proteins at 33°C. All cells expressed mRNA of cathepsin E, a pit cell marker. Moreover, transepithelial resistance was observed between apical and basolateral sides in the cells. RGE1-22 cells produced prostaglandin E₂. Levels of mRNA for cathepsin E, transepithelial resistance and prostaglandin E₂ were influenced by the nonpermissive temperature. Thus, these conditionally immortalized gastric cell lines which preserve some epithelial cell characteristics will provide a useful in vitro model of gastric epithelium.<br>

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 27 (2), 71-79, 2002

  日本細胞生物学会

キーワード

• temperature-sensitive simian virus 40 large T-antigen
• transgenic rat
• gastric epithelial cell lines
• cathepsin E
• prostaglandin E₂

詳細情報

• CRID: 1390001204695656192
• NII論文ID: 130004137438
• NII論文ID: 30023110183
• NII論文ID: 50000573558
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.27.71
• COI: 1:CAS:528:DC%2BD38Xls1Cru7Y%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 6205830
• PubMed: 12207048
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6205830
• Web Site: http://www.jstage.jst.go.jp/article/csf/27/2/27_2_71/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003006241
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可