Emotional Stress Enhanced Ghrelin Secretion from the Stomach

• Nishizawa Toshihiro

  Department of Internal Medicine, Keio University School of Medicine

• Suzuki Hidekazu

  Department of Internal Medicine, Keio University School of Medicine Department of Gastroenterology, Kitasato Institute Hospital

• Masaoka Tatsuhiro

  Department of Emergency Medicine, Keio University School of Medicine

• Nomoto Yohei

  Center for Integrated Medical Research, Keio University School of Medicine

• Minegishi Yuriko

  Center for Integrated Medical Research, Keio University School of Medicine

• Hosoda Hiroshi

  Department of Biochemistry, National Cardiovascular Center Research Institute

• Mori Mikiji

  Department of Internal Medicine, Keio University School of Medicine

• Ohara Tadashi

  Department of Internal Medicine, Tokyo Dental College

• Morishita Tetsuo

  Department of Internal Medicine, Tokyo Dental College

• Kangawa Kenji

  Department of Biochemistry, National Cardiovascular Center Research Institute

• Hibi Toshifumi

  Department of Internal Medicine, Keio University School of Medicine

抄録

Ghrelin, a novel growth hormone releasing peptide, has been reported to accelerate food intake and gastrointestinal motility. The relationship between ghrelin secretion and emotional stress remains unknown. The present study was designed to investigate ghrelin dynamics in mice after the application of the emotional stress. To apply emotional stress in the mouse model, the communication box paradigm was used. Just after the communication box protocol, the mice were examined. The plasma and gastric ghrelin levels were measured by enzyme immunoassay and radioimmunoassay. Ghrelin immunohistochemistry and quantitative RT-PCR of preproghrelin mRNA in the stomach were performed. The plasma ghrelin levels were significantly increased in the stress-applied mice. Gastric ghrelin levels and the density of ghrelin-immunoreactive cells in the stress-applied mice tended to be decreased as compared with that in the control mice, but the difference was not statistically significant. There was no significant difference in the preproghrelin mRNA expression levels between the stress-applied mice and the control mice. The present study demonstrated for the first time that the plasma level of ghrelin increased in the association emotional stress.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 38 (1), 33-37, 2006

  一般社団法人　日本酸化ストレス学会

キーワード

• ghrelin
• emotional stress
• communication box
• preproghrelin
• gastric mucosa

詳細情報

• CRID: 1390282679650749696
• NII論文ID: 130004466440
• NII論文ID: 50006223894
• DOI: 10.3164/jcbn.38.33
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://search.jamas.or.jp/link/ui/2007063829
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可