Development of the dorsal ramus of the spinal nerve in the mouse embryo: Involvement of semaphorin 3<scp>A</scp> in dorsal muscle innervation

• Tomoyuki Masuda

  Department of Histology and Neurobiology Dokkyo Medical University School of Medicine Tochigi Japan

• Masahiko Taniguchi

  Department of Molecular Medical Sciences, Research Institute for Frontier Medicine Sapporo Medical University School of Medicine Hokkaido Japan

• Chie Sakuma

  Department of Anatomy Fukushima Medical University School of Medicine Fukushima Japan

• Toshiyuki Yamagishi

  Department of Anatomy, Graduate School of Medicine Osaka City University Osaka Japan

• Shuichi Ueda

  Department of Histology and Neurobiology Dokkyo Medical University School of Medicine Tochigi Japan

• Masahumi Kawaguchi

  Department of Ultrastructural Research National Institute of Neuroscience, National Center of Neurology and Psychiatry Tokyo Japan

• Hiroyuki Yaginuma

  Department of Anatomy Fukushima Medical University School of Medicine Fukushima Japan

抄録

The spinal nerve, which is composed of dorsal root ganglion (<jats:styled-content style="fixed-case">DRG ) sensory axons and spinal motor axons, forms the dorsal ramus projecting to the dorsal musculature. By using the free‐floating immunohistochemistry method, we closely examined the spatiotemporal pattern of the formation of the dorsal ramus and the relationship between its projection to the myotome/dorsal musculature and semaphorin 3<jats:styled-content style="fixed-case">A (<jats:styled-content style="fixed-case">Sema3A ), which is an axonal guidance molecule. In embryonic day (<jats:styled-content style="fixed-case">E ) 10.5–<jats:styled-content style="fixed-case">E 11.5 wild‐type mouse embryos, we clearly showed the existence of a waiting period for the dorsal ramus projection to the myotome. In contrast, in <jats:styled-content style="fixed-case">E 10.5–<jats:styled-content style="fixed-case">E 11.5 <jats:styled-content style="fixed-case">Sema3A ‐deficient embryos, the dorsal ramus fibers projected beyond the edge of the myotome without exhibiting the waiting period for projection. These results strongly suggest that the delayed innervation by dorsal ramus fibers may be caused by Sema3A‐induced axon repulsion derived from the myotome. Next, by performing culture experiments, we confirmed that <jats:styled-content style="fixed-case">E 12.5 mouse axons responded to <jats:styled-content style="fixed-case">Sema3A ‐induced repulsion. Together, our results imply that <jats:styled-content style="fixed-case">Sema3A may play a key role in the proper development of the dorsal ramus projection.

収録刊行物

• Congenital Anomalies

  Congenital Anomalies 53 (3), 122-126, 2013-09

  Wiley

キーワード

• Developmental Biology
• Embryology
• General Medicine
• Pediatrics, Perinatology and Child Health

詳細情報

• CRID: 1360566396323853824
• NII論文ID: 50008967344
• DOI: 10.1111/cga.12019
• ISSN: 17414520
• ISSN: 09143505
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fcga.12019
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/cga.12019
• Web Site: https://search.jamas.or.jp/link/ui/2014280696
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