Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors

DOI Web Site 被引用文献34件
  • David T. Burke
    Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110.
  • Georges F. Carle
    Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110.
  • Maynard V. Olson
    Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110.

抄録

<jats:p>Fragments of exogenous DNA that range in size up to several hundred kilobase pairs have been cloned into yeast by ligating them to vector sequences that allow their propagation as linear artificial chromosomes. Individual clones of yeast and human DNA that have been analyzed by pulsed-field gel electrophoresis appear to represent faithful replicas of the source DNA. The efficiency with which clones can be generated is high enough to allow the construction of comprehensive libraries from the genomes of higher organisms. By offering a tenfold increase in the size of the DNA molecules that can be cloned into a microbial host, this system addresses a major gap in existing experimental methods for analyzing complex DNA sources.</jats:p>

収録刊行物

  • Science

    Science 236 (4803), 806-812, 1987-05-15

    American Association for the Advancement of Science (AAAS)

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