Primary Structure And Functional Expression of the 5HT ₃ Receptor, A Serotonin-gated Ion Channel

• Andres V. Maricq

  Department of Pharmacology, University of California, San Francisco, CA 94143-0450.

• Andrew S. Peterson

  Department of Physiology, University of California, San Francisco, CA 94143 0444.

• Anthony J. Brake

  Department of Pharmacology, University of California, San Francisco, CA 94143-0450.

• Richard M. Myers

  Departments of Physiology and Biochemistry and Biophysics, University of California, San Francisco, CA 94143-0444.

• David Julius

  Department of Pharmacology, University of California, San Francisco, CA 94143-0450.

抄録

The neurotransmitter serotonin (5HT) activates a variety of second messenger signaling systems and through them indirectly regulates the function of ion channels. Serotonin also activates ion channels directly, suggesting that it may also mediate rapid, excitatory responses. A complementary DNA clone containing the coding sequence of one of these rapidly responding channels, a 5HT 3 subtype of the serotonin receptor, has been isolated by screening a neuroblastoma expression library for functional expression of serotonin-gated currents in Xenopus oocytes. The predicted protein product has many of the features shared by other members of the ligand-gated ion channel family. The pharmacological and electrophysiological characteristics of the cloned receptor are largely consistent with the properties of native 5HT 3 receptors. Messenger RNA encoding this receptor is found in the brain, spinal cord, and heart. This receptor defines a new class of excitatory ligand-gated channels.

収録刊行物

• Science

  Science 254 (5030), 432-437, 1991-10-18

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1363107369653972608
• NII論文ID: 80006181441
• DOI: 10.1126/science.1718042
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.1718042
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