Expression Cloning of an Adenylate Cyclase-Coupled Calcitonin Receptor

DOI Web Site 被引用文献20件
  • Herbert Y. Lin
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
  • Tonja L. Harris
    Department of Medicine, Harvard Medical School and Medical Services (Arthritis Unit), Massachusetts General Hospital, Boston, MA 02114.
  • Merilee S. Flannery
    Department of Medicine, Harvard Medical School and Medical Services (Arthritis Unit), Massachusetts General Hospital, Boston, MA 02114.
  • Alejandro Aruffo
    Department of Medicine, Harvard Medical School and Medical Services (Arthritis Unit), Massachusetts General Hospital, Boston, MA 02114.
  • Eugene H. Kaji
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
  • Alan Gorn
    Department of Medicine, Harvard Medical School and Medical Services (Arthritis Unit), Massachusetts General Hospital, Boston, MA 02114.
  • Lee F. Kolakowski
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Harvey F. Lodish
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
  • Steven R. Goldring
    Department of Medicine, Harvard Medical School and Medical Services (Arthritis Unit), Massachusetts General Hospital, Boston, MA 02114.

抄録

<jats:p> A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant <jats:italic>K</jats:italic> <jats:sub>d</jats:sub> ≈ 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors. </jats:p>

収録刊行物

  • Science

    Science 254 (5034), 1022-1024, 1991-11-15

    American Association for the Advancement of Science (AAAS)

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