Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide

DOI Web Site 被引用文献158件
  • Peter E. Nielsen
    Research Center for Medical Biotechnology, Department of Biochemistry B, The Panum Institute, Blegdamsvej 3c, DK-2200 N, Copenhagen, Denmark.
  • Michael Egholm
    Department of Organic Chemistry, The H. C. Ørsted Institute, Universitetsparken 5, DK-2100 Ø, Copenhagen, Denmark.
  • Rolf H. Berg
    Polymer Group, Materials Department, Risø National Laboratory, DK-4000 Roskilde, Denmark.
  • Ole Buchardt
    Department of Organic Chemistry, The H. C. Ørsted Institute, Universitetsparken 5, DK-2100 Ø, Copenhagen, Denmark.

抄録

<jats:p>A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.</jats:p>

収録刊行物

  • Science

    Science 254 (5037), 1497-1500, 1991-12-06

    American Association for the Advancement of Science (AAAS)

被引用文献 (158)*注記

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