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- 浜野 英也
- 東京医科歯科大学歯学部第2歯科補綴学教室 東京医科歯科大学歯学部第2歯科理工学教室
書誌事項
- タイトル別名
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- Fundamental Studies on Biological Effects of Dental Metals. Nickel Dissolution, Toxicity and Distribution in Cultured Cells.
- Nickel Dissolution, Toxicity and Distribution in Cultured Cells
- ニッケルの溶出ならびに培養細胞における毒性と経時的動態
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抄録
In an oral environment, minute amounts of constituent metals dissolve from the surfaces of restorations. The uptake of the metals through the mucosa may cause systemic or local effects. The purpose of this study was to investigate these effects and mechanisms, focusing on the nickel as a dental metal. <BR>A nickel-chromium dental casting alloy was stored in 3 immersion solutions for 4 weeks and the amount of nickel dissolved was measured. NiC12 was administrated to the cultured medium to examine the cytotoxicity for HeLa cells and periodontal ligamentderived cells as well as the nickel distribution in the HeLa cells. <BR>The results were as follows: <BR>1. The amounts of nickel released from the alloy were 1.2. μg/cm2 (human saliva), 2.0. μg/cm2 (PBS (-) ) and 2.5. μg/cm2 (MILLI-Q water) . <BR>2. 3.0mM NiCl2 showed a large cytotoxicity resulting in the cell growth rate and morphological changes. <BR>3. Incubated for 72 hours in 0.15mM NiCl2, the amount of nickel in the 106 cells was 20 ng. After 24 hours of incubation with NiCl2, and the following 72 hours without NiCl2, the amount of nickel had decreased, but 30% still remained. <BR>4. Incubated for 72 hours in 0.15mM NiCl2, 65% of the nickel in the cells was bound by the soluble fraction. <BR>These results suggest that nickel released from the oral prosthesis may be sequestered for a long term in the cells and possibly causes some effects.
収録刊行物
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- 口腔病学会雑誌
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口腔病学会雑誌 59 (2), 456-478, 1992
口腔病学会