書誌事項
- タイトル別名
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- ANALYSIS OF IgG SUBCLASS IN FACTOR Ⅷ INHIBITOR BY ENZYME-LINKED IMMUNOSORBENT ASSAY
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type:Article
Semi-quantitative analysis for factor Ⅷ (F. Ⅷ)-specific IgG subclass in the plasma of hemophilia A with F. Ⅷ inhibitor was developed by enzyme-linked immunosorbent assay (ELISA) using immobilized recombinant F. Ⅷ. The bound F. Ⅷ-specific IgG subclass was detected by monoclonal anti-IgG₁, IgG₂, IgG₃ and IgG₄ antibodies followed by peroxidase conjugated goat anti-mouse IgG. Out of 82 plasma samples from 14 patients with inhibitor, 53 samples from 11 patients were positive for only IgG₄. There was no sample which was positive for only IgG₁, IgG₂ or IgG₃. Of the eleven patients with samples which were positive for only IgG₄, one patient was also positive for only IgG₁ in one sample, four patients were also positive for only IgG₂ in eight samples and one patient was positive for only IgG₃ in three samples on different dates. None of F. Ⅷ-specific IgG subclass was detected in the plasma from 27 samples of hemophilia A without inhibitor. In conclusion, the ELISA system was suitable not only for the detection of F. Ⅷ inhibitor but also for semi-quantitative estimation of changes in the plasma content of F. Ⅷ-specific IgG subclass. Furthermore, it was demonstrated that the dominant subclass among the inhibitors was IgG₄. However, other IgG subclasses were also detected in some cases.
identifier:奈良医学雑誌 Vol.43 No.5 p.511-521
identifier:04695550
identifier:13450069
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1867
identifier:奈良医学雑誌, 43(5): 511-521
収録刊行物
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- 奈良医学雑誌
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奈良医学雑誌 43 (5), 511-521, 1992-10-31
奈良医学会
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詳細情報
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- CRID
- 1050564287448566656
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- NII論文ID
- 80006910946
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- NII書誌ID
- AN00180974
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- ISSN
- 04695550
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- 本文言語コード
- ja
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- CiNii Articles