Monocyte chemotaxis by the 96 well assembly. One step quantitative measurement by the micro-plate reader.

DOI 9 Citations

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  • ９６穴ケモタキシスチャンバーを用いた白血球走化性試験　マイクロプレートリーダーによる走化性の定量的評価
  • -<I>One step quantitative measurement by the micro-plate reader</I>
  • ―マイクロプレートリーダーによる走化性の定量的評価―

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Abstract

A new multi-well assembly was used for the in vitro chemotaxis assay of human peripheral blood monocytes. N-formylmethionyl-leucyl-phenylalanine caused concentration-dependent migration of the monocyte into the lower surface of the metal flamed membrane filter. Measurement was performed by the microplate reader (550-600 nm), and the absorbance showed the complete dependence on the number of migrated cells.

Journal

  • Ensho

    Ensho 13 (1), 51-54, 1993

    The Japanese Society of Inflammation and Regeneration

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