Cyclin D1 is a nuclear protein required for cell cycle progression in G1.

• V Baldin

• J Lukas

• M J Marcote

• M Pagano

• G Draetta

抄録

A cascade of events is triggered upon the addition of growth factor to quiescent mammalian cells, which ultimately restarts proliferation by inducing the transition from G0/G1 to S-phase. We have studied cyclin D1, a putative G1 cyclin, in normal diploid human fibroblasts. Cyclin D1 accumulated and reached a maximum level before S-phase upon the addition of serum to quiescent cells. The protein was localized to the nucleus, and it disappeared from the nucleus as cells proceeded into S-phase. Microinjection of anti-cyclin D1 antibodies or antisense plasmid prevented cells from entering S-phase, and the kinetics of inhibition showed that cyclin D1 is required at a point in the cell cycle earlier than cyclin A. These results demonstrate that cyclin D1 is a critical target of proliferative signals in G1.

収録刊行物

• Genes & Development

  Genes & Development 7 (5), 812-821, 1993-05

  Cold Spring Harbor Laboratory

キーワード

• Developmental Biology
• Genetics

詳細情報

• CRID: 1360855569775438464
• NII論文ID: 80007106926
• DOI: 10.1101/gad.7.5.812
• ISSN: 15495477
• ISSN: 08909369
• ISSN: http://id.crossref.org/issn/08909369
• Web Site: https://syndication.highwire.org/content/doi/10.1101/gad.7.5.812
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