Green Fluorescent Protein as a Marker for Gene Expression

DOI Web Site 被引用文献222件
  • Martin Chalfie
    Department of Biological Sciences, Columbia University, New York, NY 10027.
  • Yuan Tu
    Department of Biological Sciences, Columbia University, New York, NY 10027.
  • Ghia Euskirchen
    Department of Biological Sciences, Columbia University, New York, NY 10027.
  • William W. Ward
    Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, NJ 08903, USA.
  • Douglas C. Prasher
    Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.

抄録

<jats:p> A complementary DNA for the <jats:italic>Aequorea victoria</jats:italic> green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic ( <jats:italic>Escherichia coli</jats:italic> ) or eukaryotic ( <jats:italic>Caenorhabditis elegans</jats:italic> ) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms. </jats:p>

収録刊行物

  • Science

    Science 263 (5148), 802-805, 1994-02-11

    American Association for the Advancement of Science (AAAS)

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