Salt-induced formation of the molten globule state of cytochrome c studied by isothermal titration calorimetry.
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- D Hamada
- Department of Biology, Faculty of Science, Osaka University, Japan.
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- S Kidokoro
- Department of Biology, Faculty of Science, Osaka University, Japan.
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- H Fukada
- Department of Biology, Faculty of Science, Osaka University, Japan.
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- K Takahashi
- Department of Biology, Faculty of Science, Osaka University, Japan.
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- Y Goto
- Department of Biology, Faculty of Science, Osaka University, Japan.
抄録
<jats:p>Although the molten globule state has been proposed as a major intermediate of protein folding, it has proven difficult to obtain thermodynamic data characterizing this state. To explore another approach for characterizing the molten globule state, salt-induced formation of the molten globule state of horse cytochrome c at pH 1.8 was studied by isothermal titration calorimetry. By titrating the acid-unfolded cytochrome c with sodium perchlorate, an exothermic reaction was observed. The titration curve obtained from the heat was cooperative and agreed well with the conformational transition curve measured by CD at 222 nm. This result indicated that the salt-induced conformation change is well approximated by a two-state transition between the acid-unfolded and molten globule states. The heat for formation of the molten globule state estimated by isothermal titration calorimetry was consistent with the enthalpy change for unfolding of the sodium perchlorate-stabilized molten globule state at pH 1.8, which was measured by differential scanning calorimetry and CD. These results indicate that the heat of titration largely reflects the enthalpy change of the conformational transition. From these results, we consider that isothermal titration calorimetry will become a useful approach for investigating the molten globule state.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 91 (22), 10325-10329, 1994-10-25
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1360574095149702144
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- NII論文ID
- 80007933900
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- ISSN
- 10916490
- 00278424
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- データソース種別
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