Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.
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- C Nawrath
- Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305.
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- Y Poirier
- Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305.
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- C Somerville
- Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305.
抄録
<jats:p>In the bacterium Alcaligenes eutrophus, three genes encode the enzymes necessary to catalyze the synthesis of poly[(R)-(-)-3-hydroxybutyrate] (PHB) from acetyl-CoA. In order to target these enzymes into the plastids of higher plants, the genes were modified by addition of DNA fragments encoding a pea chloroplast transit peptide, a constitutive plant promoter, and a poly(A) addition sequence. Each of the modified bacterial genes was introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation, and plants containing all three genes were obtained by sexual crosses. These plants accumulated PHB up to 14% of the dry weight as 0.2- to 0.7-micron granules within plastids. In contrast to earlier experiments in which expression of the PHB biosynthetic pathway in the cytoplasm led to a deleterious effect on growth, expression of the PHB biosynthetic pathway in plastids had no obvious effect on the growth or fertility of the transgenic plants and resulted in a 100-fold increase in the amount of PHB that accumulated. We conclude that there does not appear to be any biological barrier to high-level production of PHB in higher plants. The high level of PHB accumulation also suggests that the synthesis of plastid acetyl-CoA is regulated by a mechanism which responds to metabolic demand.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 91 (26), 12760-12764, 1994-12-20
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1362544420921162112
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- NII論文ID
- 80008081835
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- ISSN
- 10916490
- 00278424
- http://id.crossref.org/issn/00278424
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