Visualization of protein transport along the secretory pathway using green fluorescent protein

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<jats:p>We have expressed green fluorescent protein (GFP) from <jats:italic>A. victoria</jats:italic> in the secretory pathway of HeLa cells by fusing it to the C‐terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37°C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was observed when biosynthetic protein transport was arrested at the intermediate compartment or the <jats:italic>trans</jats:italic>‐Golgi network by temperature blocks (15°C and 20°C, respectively). Reversal of the temperature blocks allowed the visualization of secretion of fluorescent GFP and offers the possibility to analyse transport in the secretory pathway in living cells.</jats:p>

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  • FEBS Letters

    FEBS Letters 369 (2-3), 267-271, 1995-08-07

    Wiley

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