The RNA Component of Human Telomerase

• Junli Feng

  Geron Corporation, Menlo Park, CA 94025, USA.

• Walter D. Funk

  Geron Corporation, Menlo Park, CA 94025, USA.

• Sy-Shi Wang

  Geron Corporation, Menlo Park, CA 94025, USA.

• Scott L. Weinrich

  Geron Corporation, Menlo Park, CA 94025, USA.

• Ariel A. Avilion

  Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

• Choy-Pik Chiu

  Geron Corporation, Menlo Park, CA 94025, USA.

• Robert R. Adams

  Geron Corporation, Menlo Park, CA 94025, USA.

• Edwin Chang

  Geron Corporation, Menlo Park, CA 94025, USA.

• Richard C. Allsopp

  Geron Corporation, Menlo Park, CA 94025, USA.

• Jinghua Yu

  Geron Corporation, Menlo Park, CA 94025, USA.

• Siyuan Le

  Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

• Michael D. West

  Geron Corporation, Menlo Park, CA 94025, USA.

• Calvin B. Harley

  Geron Corporation, Menlo Park, CA 94025, USA.

• William H. Andrews,

  Geron Corporation, Menlo Park, CA 94025, USA.

• Carol W. Greider

  Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

• Bryant Villeponteau

  Geron Corporation, Menlo Park, CA 94025, USA.

抄録

Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5′-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG) n . Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.

収録刊行物

• Science

  Science 269 (5228), 1236-1241, 1995-09

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360855571004894208
• NII論文ID: 80008542634
• DOI: 10.1126/science.7544491
• ISSN: 10959203
• ISSN: 00368075
• ISSN: http://id.crossref.org/issn/00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.7544491
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  • CiNii Articles