A detergent-free method for purifying caveolae membrane from tissue culture cells.

DOI Web Site 被引用文献17件
  • E J Smart
    Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235, USA.
  • Y S Ying
    Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235, USA.
  • C Mineo
    Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235, USA.
  • R G Anderson
    Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235, USA.

抄録

<jats:p>Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.</jats:p>

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