Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.
-
- M Schena
- Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu
-
- D Shalon
- Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu
-
- R Heller
- Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu
-
- A Chai
- Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu
-
- P O Brown
- Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu
-
- R W Davis
- Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu
抄録
<jats:p>Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.</jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 93 (20), 10614-10619, 1996-10
Proceedings of the National Academy of Sciences
- Tweet
キーワード
詳細情報
-
- CRID
- 1363670318870550656
-
- NII論文ID
- 80009261505
-
- ISSN
- 10916490
- 00278424
-
- データソース種別
-
- Crossref
- CiNii Articles