Specific Oxidative Cleavage of Carotenoids by VP14 of Maize

Steven H. Schwartz, Bao Cai Tan, Douglas A. Gage, Jan A. D. Zeevaart, Donald R. McCarty

doi:10.1126/science.276.5320.1872

Specific Oxidative Cleavage of Carotenoids by VP14 of Maize

DOI Web Site 被引用文献45件

Steven H. Schwartz

S. H. Schwartz and J. A. D. Zeevaart, Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Bao Cai Tan

S. H. Schwartz and J. A. D. Zeevaart, Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Douglas A. Gage

S. H. Schwartz and J. A. D. Zeevaart, Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Jan A. D. Zeevaart

S. H. Schwartz and J. A. D. Zeevaart, Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Donald R. McCarty

S. H. Schwartz and J. A. D. Zeevaart, Michigan State University–Department of Energy (MSU-DOE) Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.

抄録

<jats:p> The plant growth regulator abscisic acid (ABA) is formed by the oxidative cleavage of an epoxy-carotenoid. The synthesis of other apocarotenoids, such as vitamin A in animals, may occur by a similar mechanism. In ABA biosynthesis, oxidative cleavage is the first committed reaction and is believed to be the key regulatory step. A new ABA-deficient mutant of maize has been identified and the corresponding gene, <jats:italic>Vp14</jats:italic> , has been cloned. The recombinant VP14 protein catalyzes the cleavage of 9- <jats:italic>cis</jats:italic> -epoxy-carotenoids to form C <jats:sub>25</jats:sub> apo-aldehydes and xanthoxin, a precursor of ABA in higher plants. </jats:p>