Chemokines and the Arrest of Lymphocytes Rolling Under Flow Conditions

• James J. Campbell

  J. J. Campbell and E. C. Butcher, Laboratory of Immunology and Vascular Biology, Department of Pathology, and Digestive Disease Center, Department of Medicine, Stanford University Medical School, Stanford, CA 94305, USA, and Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA.

• Joseph Hedrick

  J. J. Campbell and E. C. Butcher, Laboratory of Immunology and Vascular Biology, Department of Pathology, and Digestive Disease Center, Department of Medicine, Stanford University Medical School, Stanford, CA 94305, USA, and Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA.

• Albert Zlotnik

  J. J. Campbell and E. C. Butcher, Laboratory of Immunology and Vascular Biology, Department of Pathology, and Digestive Disease Center, Department of Medicine, Stanford University Medical School, Stanford, CA 94305, USA, and Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA.

• Michael A. Siani

  J. J. Campbell and E. C. Butcher, Laboratory of Immunology and Vascular Biology, Department of Pathology, and Digestive Disease Center, Department of Medicine, Stanford University Medical School, Stanford, CA 94305, USA, and Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA.

• Darren A. Thompson

  J. J. Campbell and E. C. Butcher, Laboratory of Immunology and Vascular Biology, Department of Pathology, and Digestive Disease Center, Department of Medicine, Stanford University Medical School, Stanford, CA 94305, USA, and Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA.

• Eugene C. Butcher

  J. J. Campbell and E. C. Butcher, Laboratory of Immunology and Vascular Biology, Department of Pathology, and Digestive Disease Center, Department of Medicine, Stanford University Medical School, Stanford, CA 94305, USA, and Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA.

Abstract

Circulating lymphocytes are recruited from the blood to the tissue by rolling along the endothelium until being stopped by a signaling event linked to the G i α subunit of a heterotrimeric GTP-binding protein; that event then triggers rapid integrin-dependent adhesion. Four chemokines are now shown to induce such adhesion to intercellular adhesion molecule–1 and to induce arrest of rolling cells within 1 second under flow conditions similar to those of blood. SDF-1 (also called PBSF), 6-C-kine (also called Exodus-2), and MIP-3β (also called ELC or Exodus-3) induced adhesion of most circulating lymphocytes, including most CD4 + T cells; and MIP-3α (also called LARC or Exodus-1) triggered adhesion of memory, but not naı̈ve, CD4 + T cells. Thus, chemokines can regulate the arrest of lymphocyte subsets under flowing conditions, which may allow them to control lymphocyte–endothelial cell recognition and lymphocyte recruitment in vivo.

Journal

• Science

  Science 279 (5349), 381-384, 1998-01-16

  American Association for the Advancement of Science (AAAS)

Keywords

• Multidisciplinary

Details

• CRID: 1360292621561453824
• NII Article ID: 80010090882
• DOI: 10.1126/science.279.5349.381
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.279.5349.381
• Data Source:
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  • CiNii Articles