A simplified system for generating recombinant adenoviruses

DOI Web Site 被引用文献45件
  • Tong-Chuan He
    The Howard Hughes Medical Institute, The Program in Human Genetics and Molecular Biology, The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231
  • Shibin Zhou
    The Howard Hughes Medical Institute, The Program in Human Genetics and Molecular Biology, The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231
  • Luis T. da Costa
    The Howard Hughes Medical Institute, The Program in Human Genetics and Molecular Biology, The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231
  • Jian Yu
    The Howard Hughes Medical Institute, The Program in Human Genetics and Molecular Biology, The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231
  • Kenneth W. Kinzler
    The Howard Hughes Medical Institute, The Program in Human Genetics and Molecular Biology, The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231
  • Bert Vogelstein
    The Howard Hughes Medical Institute, The Program in Human Genetics and Molecular Biology, The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231

抄録

<jats:p>Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.</jats:p>

収録刊行物

被引用文献 (45)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ