Cloning of the <i>Arabidopsis</i> <i>ent</i> -kaurene oxidase gene <i> GA <i>3</i> </i>

DOI Web Site 被引用文献18件
  • Chris A. Helliwell
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom CB2 3EA; Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312
  • Candice C. Sheldon
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom CB2 3EA; Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312
  • Mark R. Olive
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom CB2 3EA; Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312
  • Amanda R. Walker
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom CB2 3EA; Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312
  • Jan A. D. Zeevaart
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom CB2 3EA; Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312
  • W. James Peacock
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom CB2 3EA; Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312
  • Elizabeth S. Dennis
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, United Kingdom CB2 3EA; Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312

抄録

<jats:p> The <jats:italic>ga3</jats:italic> mutant of <jats:italic>Arabidopsis</jats:italic> is a gibberellin-responsive dwarf. We present data showing that the <jats:italic>ga3-1</jats:italic> mutant is deficient in <jats:italic>ent-</jats:italic> kaurene oxidase activity, the first cytochrome P450-mediated step in the gibberellin biosynthetic pathway. By using a combination of conventional map-based cloning and random sequencing we identified a putative cytochrome P450 gene mapping to the same location as <jats:italic>GA3</jats:italic> . Relative to the progenitor line, two <jats:italic>ga3</jats:italic> mutant alleles contained single base changes generating in-frame stop codons in the predicted amino acid sequence of the P450. A genomic clone spanning the P450 locus complemented the <jats:italic>ga3-2</jats:italic> mutant. The deduced GA3 protein defines an additional class of cytochrome P450 enzymes. The <jats:italic>GA3</jats:italic> gene was expressed in all tissues examined, RNA abundance being highest in inflorescence tissue. </jats:p>

収録刊行物

被引用文献 (18)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ