Degradation of Misfolded Endoplasmic Reticulum Glycoproteins in <i>Saccharomyces cerevisiae</i> Is Determined by a Specific Oligosaccharide Structure

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  • Claude A. Jakob
    *Division of Cell and Molecular Pathology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and ‡Mikrobiologisches Institut, ETH Zürich, CH-8092 Zürich, Switzerland
  • Patricie Burda
    *Division of Cell and Molecular Pathology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and ‡Mikrobiologisches Institut, ETH Zürich, CH-8092 Zürich, Switzerland
  • Jürgen Roth
    *Division of Cell and Molecular Pathology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and ‡Mikrobiologisches Institut, ETH Zürich, CH-8092 Zürich, Switzerland
  • Markus Aebi
    *Division of Cell and Molecular Pathology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland; and ‡Mikrobiologisches Institut, ETH Zürich, CH-8092 Zürich, Switzerland

抄録

<jats:p>In Saccharomyces cerevisiae, transfer of N-linked oligosaccharides is immediately followed by trimming of ER-localized glycosidases. We analyzed the influence of specific oligosaccharide structures for degradation of misfolded carboxypeptidase Y (CPY). By studying the trimming reactions in vivo, we found that removal of the terminal α1,2 glucose and the first α1,3 glucose by glucosidase I and glucosidase II respectively, occurred rapidly, whereas mannose cleavage by mannosidase I was slow. Transport and maturation of correctly folded CPY was not dependent on oligosaccharide structure. However, degradation of misfolded CPY was dependent on specific trimming steps. Degradation of misfolded CPY with N-linked oligosaccharides containing glucose residues was less efficient compared with misfolded CPY bearing the correctly trimmed Man8GlcNAc2 oligosaccharide. Reduced rate of degradation was mainly observed for mis- folded CPY bearing Man6GlcNAc2, Man7GlcNAc2 and Man9GlcNAc2 oligosaccharides, whereas Man8GlcNAc2 and, to a lesser extent, Man5GlcNAc2 oligosaccharides supported degradation. These results suggest a role for the Man8GlcNAc2 oligosaccharide in the degradation process. They may indicate the presence of a Man8GlcNAc2-binding lectin involved in targeting of misfolded glycoproteins to degradation in S. cerevisiae.</jats:p>

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