Identification of Δ12‐fatty acid desaturase from arachidonic acid‐producing <i>Mortierella</i> fungus by heterologous expression in the yeast <i>Saccharomyces cerevisiae</i> and the fungus <i>Aspergillus oryzae</i>

• Eiji Sakuradani

• Michihiko Kobayashi

• Toshihiko Ashikari

• Sakayu Shimizu

抄録

Based on the sequence information for the ω3‐desaturase genes (from Brassica napus and Caenorhabditis elegans ), which are involved in the desaturation of linoleic acid (Δ9, Δ12–18 : 2) to α‐linolenic acid (Δ9, Δ12, Δ15–18 : 3), a cDNA was cloned from the filamentous fungal strain, Mortierella alpina 1S‐4, which is used industrially to produce arachidonic acid. Homology analysis with protein databases revealed that the amino acid sequence showed 43.7% identity as the highest match with the microsomal ω6‐desaturase (from Glycine max , soybean), whereas it exhibited 38.9% identity with the microsomal ω3‐desaturase (from soybean). The evolutionary implications of these enzymes will be discussed. The cloned cDNA was confirmed to encode a Δ12‐desaturase, which was involved in the desaturation of oleic acid (Δ9–18 : 1) to linoleic acid, by its expression in both the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae . Analysis of the fatty acid composition of yeast and fungus transformants demonstrated that linoleic acid (which was not contained in the control strain of S .  cerevisiae ) was accumulated in the yeast transformant and that the fungal transformant contained a large amount of linoleic acid (71.9%). Genomic Southern blot analysis of the transformants with the Mortierella Δ12‐desaturase gene as a probe confirmed integration of this gene into the genome of A. oryzae . The M .  alpina 1S‐4 Δ12‐desaturase is the first example of a cloned nonplant Δ12‐desaturase.

収録刊行物

• European Journal of Biochemistry

  European Journal of Biochemistry 261 (3), 812-820, 1999-05

  Wiley

キーワード

• Biochemistry

詳細情報

• CRID: 1363388845161471488
• NII論文ID: 80011098210
• DOI: 10.1046/j.1432-1327.1999.00333.x
• ISSN: 14321033
• ISSN: 00142956
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.1999.00333.x
• Web Site: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.1999.00333.x
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